We narrowed to 9,039 results for: CAG

1. pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

   Plasmid

   #84250

   Purpose

   Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sa sgTRE3G
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATG9A sgRNA

   Plasmid

   #207557

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CACTGAATACCAGCGCCTAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MK1283

   Plasmid

   #71428

   Purpose

   This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

   Depositor

   Howard Salis

   Article

   Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

   Insert

   Mixed Feedback Loop version of the UBER system
   

   Use

   Tags

   Expression

   Mutation

   T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

   Promoter

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. WIPI2 sgRNA

   Plasmid

   #207554

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CGCGCGCCCAGCCATGAACC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. VPS35 shRNA2

   Plasmid

   #163859

   Purpose

   shRNA targeting the coding sequences of VPS35

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   VPS35 retromer complex component (VPS35 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-shRNA-Tet2

   Plasmid

   #85743

   Purpose

   shRNA against mouse Tet2

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   shRNA Tet2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniCMV-iRFP670-tDeg

   Plasmid

   #185403

   Purpose

   iRFP670-tDeg fluorogenic protein

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

   Insert

   iRFP670-tDeg
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR - AAVS1 sgRNA

    Plasmid

    #70661

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against AAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. CMV-VARNAM

    Plasmid

    #115552

    Purpose

    Red fluorescent voltage indicator for mammalian expression

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

    Insert

    VARNAM
    

    Use

    Tags

    Golgi and ER export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATG16L1 sgRNA

    Plasmid

    #207563

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    GCAGCAAGTGACATGTCGTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-hNoxa1

    Plasmid

    #58531

    Purpose

    expresses human Noxa1 in mammalian cells

    Depositor

    Botond Banfi, Karl-Heinz Krause

    Article

    Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

    Insert

    NADPH oxidase activator 1 (NOXA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-NEAT1pr_v2

    Plasmid

    #97082

    Purpose

    Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1pr_v2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet1-sgRNA(siteT9)

    Plasmid

    #154832

    Purpose

    sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    sgRNA(siteT9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-blast shKGA

    Plasmid

    #110420

    Purpose

    shKGA, silence glutaminase KGA isoform, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA Pol III)

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458 pSp Cas9 Rhino1 tagA

    Plasmid

    #211627

    Purpose

    Cas9 Rhino1 tagA

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    RHNO tagA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX-EGFP-g1

    Plasmid

    #107273

    Purpose

    eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    GFP sgRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTK73.ChrII_ttTi5605.sgRNA

    Plasmid

    #194058

    Purpose

    sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ChrII_ttTi5605 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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