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pET28CLY6

Plasmid

#21765

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pdestDD03

Plasmid

#26414

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2 cassette

Expression

Bacterial and Worm

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pStrep-2xEGFP-N1

Plasmid

#122489

Purpose

Expression of Strep-tagged 2xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 2xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR4_TAF15

Plasmid

#26368

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

TAF15 (TAF15 Human)

Use

Entry vector

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
cyterm-ymtq2 pDRF1-GW

Plasmid

#168083

Purpose

Expresses Cyterm-ymTq2 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymTq2

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP

Plasmid

#48244

Purpose

Untagged, full-length protein for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Tags

None

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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Dronpa-Mito-3

Plasmid

#57287

Purpose

Localization: Mitochondria, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

Dronpa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pNIC-PelB-NH10T-AVITAG-GG

Plasmid

#215793

Purpose

Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-GST-TEV-AVITAG-GG

Plasmid

#215809

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRSET-B-frSkyran-S

Plasmid

#182209

Purpose

Bacterial expression of frSkylan-S

Depositor

Shiro Suetsugu

Article

Osuga et al Mol Biol Cell. 2021 Nov 1;32(21):br7. do

Insert

frSkylan-S

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28CLY7

Plasmid

#21766

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pCYP15

Plasmid

#212848

Purpose

Expresses the Micromonospora cremea LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLRD12

Plasmid

#208264

Purpose

Production of miltiradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cldB
smKAL

Expression

Bacterial

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pQC NLS mKorange IX

Plasmid

#37346

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

NLS mKusabira Orange

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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GNb-SNAPf

Plasmid

#128770

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-SNAPf

Expression

Mammalian

Available Since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCVD433

Plasmid

#63756

Purpose

Derivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now Tcs

Depositor

James Kaper

Article

Donnenberg et al Infect Immun. 1990 Jun;58(6):1565-71.

Type

Empty backbone

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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2xmCherry2

Plasmid

#127804

Purpose

Homo-dimer mCherry2-mCherry2 expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mCherry2-mCherry2

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEM7070

Plasmid

#214008

Purpose

PrpsM driven expression of ssrA-tagged mNeonGreen (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen-ASV

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7074

Plasmid

#214012

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-ASV

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7068

Plasmid

#214006

Purpose

PrpsM driven expression of ssrA-tagged eGFP (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

eGFP-ASV

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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