We narrowed to 18,914 results for: Tec

1. pNICKclos1.0

   Plasmid

   #73639

   Purpose

   Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sGRNA to pyrE
   

   Use

   E.coli-clostridium shuttle vector

   Mutation

   D10A

   Promoter

   j23119 and ptb

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCH68 (dAsCas12a-3xKRAB piggyBac)

   Plasmid

   #217334

   Purpose

   piggyBac expression of dAsCas12a-3xKRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   dAsCas12a
   

   Use

   CRISPR

   Tags

   nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E993A

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115208

   Purpose

   Expresses PinCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PinCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dCpf1-BE

   Plasmid

   #107685

   Purpose

   Expresses dLbCpf1-BE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE
   

   Expression

   Mammalian

   Mutation

   D832A/E925A/D1148A

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-2xFYVE_hrs

   Plasmid

   #67676

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   2xFYVEhrs
   

   Use

   Gateway multisite 3' entry clone

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. gRNA NIA Null

   Plasmid

   #198728

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs
   

   Expression

   Plant

   Promoter

   pU6-26, pU6-29

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pv6_CBX1_2xChromo

   Plasmid

   #179393

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY2ShHELIX_sgRNA_entry (CJT30)

   Plasmid

   #181781

   Purpose

   Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Expression

   Bacterial

   Mutation

   Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-APOBEC1-YTHD422N

   Plasmid

   #194432

   Purpose

   APOBEC1 fused to YTHD422N

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTHD422N (APOBEC1 Human)
   

   Use

   CRISPR

   Tags

   APOBEC1-YTHD422N-HA

   Expression

   Mammalian

   Mutation

   D422N mutation in the YTH domain to enhance m6A b…

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. dTomato_LD 0_CS M_tTA-BFP

    Plasmid

    #58875

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB510

    Plasmid

    #74852

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115207

    Purpose

    Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    BzoCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB415

    Plasmid

    #74809

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB453

    Plasmid

    #74835

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid

    #83485

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCALNL-NaChBac

    Plasmid

    #125580

    Purpose

    Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    NaChBac
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-ZoAl-RTD-3xF-ORF

    Plasmid

    #238486

    Purpose

    For production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    DD644/645->AA

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-ZoAl-END-3xF-ORF

    Plasmid

    #238485

    Purpose

    For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    DD1041/1054->AA

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-TaGu-RTD-3xF-ORF

    Plasmid

    #238490

    Purpose

    For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    DD660/661->AA

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-TaGu-END-3xF-ORF

    Plasmid

    #238489

    Purpose

    For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    DD1057/1070->AA

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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