We narrowed to 26,401 results for: Vit

1. pLKO.1P shACO1

   Plasmid

   #169884

   Purpose

   Suppress ACO1

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   shRNA against ACO1
   

   Use

   Lentiviral and RNAi

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-mTagBFP

   Plasmid

   #75175

   Purpose

   TagBFP (blue) fluorophore with stop codon for C-terminal fusions

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   TagBFP
   

   Use

   Three prime entry vector for multisite gateway th…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS2156_Nme2-ABE8e-nt

   Plasmid

   #201503

   Purpose

   Mammalian expression of N-terminally fused Nme2-ABE8e-nt

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-nt
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM60 mUlk1 (wt) barcoded

   Plasmid

   #48799

   Purpose

   expresses wt Ulk1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   ULK1 (Ulk1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and barcode: GGATCC

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-ABKAR(T/A)

   Plasmid

   #140052

   Purpose

   Negative control mutant for ABKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR(T/A)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-mCherry-N1-ChloC

   Plasmid

   #85461

   Purpose

   Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Peter Hegemann

   Article

   Wietek et al Science. 2014 Mar 27.

   Insert

   ChloC
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E90R, T159C

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRN3P_T3_ABEmax_IVT

   Plasmid

   #171761

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   ABEmax
   

   Use

   Vector for in-vitro transcription

   Promoter

   T3 promoter

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-Gateway-DSCP

   Plasmid

   #71506

   Purpose

   flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Type

   Empty backbone

   Use

   Flystarr-seq validation vector

   Tags

   luciferase

   Expression

   Insect

   Available Since

   Dec. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT44c

   Plasmid

   #138511

   Purpose

   P2

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   inteinC-dSpCas9-UGI
   

   Use

   Synthetic Biology

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AB.pCCL.sin.cPPT.U6.miR-335-5p-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46612

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-335-5p Decoy
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. FRB-ECFP(W66A)-Giantin

    Plasmid

    #67903

    Purpose

    Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    FRB-ECFP(W66A)-Giantin
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18-H1 RNAi

    Plasmid

    #87355

    Purpose

    RNAi expression vector based on H1 promoter and pUC18 vector backbone

    Depositor

    Saroj Mathupala

    Article

    Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

    Insert

    H1 RNA Promoter from U87MG glioma cells
    

    Use

    RNAi; Shrna expression vector

    Expression

    Mammalian

    Promoter

    H1 RNA

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-hSyn-Galpha-i3 ONE-GO

    Plasmid

    #204334

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3 ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBabe-PI(WT)-Src(WT)-mCherry

    Plasmid

    #87356

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFAP-Lyn-mCherry-pHluorin

    Plasmid

    #187980

    Purpose

    Membrane targeted cytoplasmic fluorescent pH sensor

    Depositor

    Chris Dulla

    Article

    Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

    Insert

    Lyn-mcherry-pHluorin
    

    Use

    AAV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEG R2-L3 #BGV193

    Plasmid

    #48952

    Purpose

    For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. SG3ΔENV CA M66I

    Plasmid

    #149687

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Capsid residue Met 66 to Iso (CA M66I)

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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