We narrowed to 11,248 results for: ena
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Plasmid#85969PurposeUCOE-SFFV-dCas9-BFP-KRABDepositorInsertdCas9
UseLentiviralTagsBFP-KRABExpressionMammalianMutationD10A, H840APromoterSFFVAvailable SinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
SNAPtag-PTS1
Plasmid#182883PurposeExpress SNAPtag-PTS1 in mammalian cells. SNAPtag targets to peroxisomesDepositorInsertSNAPtag-PTS1
UseLentiviralTagsSNAPtagExpressionMammalianMutationnoPromoterCMVAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-B19G
Plasmid#100799Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTADepositorHas ServiceAAV1InsertmTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
UseAAVExpressionMammalianPromoterTREtightAvailable SinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-DAP5-3XFLAG
Plasmid#127331PurposeExpress 3xFLAG tagged DAP5 protein from AUG start codonDepositorAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-150-5p
Plasmid#103257PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-150-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-150-5p target (MIR150 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-SOX9
Plasmid#141404PurposeInducible Expression of SOX9DepositorAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-130b-3p
Plasmid#103217PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-130b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-130b-3p target (MIR130B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1
Plasmid#159128Purposeexpresses KEAP1 in mammalian cellsDepositorAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-TopFlash-NanoLucP
Plasmid#204347PurposeTopFlash reporterDepositorInsertTopFlash-NanoLucP
UseLuciferaseTagsNanoLucPPromoterminiPAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
ExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114103Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVExpressionMammalianPromotermotoneuron-specific Homeobox 9 promoterAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJ114
Plasmid#85995PurposeOne-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant regionDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianPromotermodified bovine U6-2Available SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-SERBP1(149-400)
Plasmid#176516PurposeExpresses the human C-terminal SERBP1 149-400 construct.DepositorInsertSERBP1 149-400 (SERBP1 Human)
TagsHis8 tag, linker, TEV siteExpressionBacterialMutationdeleted amino acids 1-148 and 401-408PromoterT7Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 MS2 sgRNA
Plasmid#92394PurposeChick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCPDepositorInsertchick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
UseCRISPRExpressionMammalianPromoterchick U6.3Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
AcGFP-MSH3
Plasmid#215122PurposeExpresses AcGFP-MSH3 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-20nm-EBFP2
Plasmid#231185PurposeExpresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nmDepositorInsert20nm-EBFP2
ExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgCTNNB1 Nicking
Plasmid#169845PurposeExpress a nicking sgRNA used for installation of the oncogenic S45F or S45del in Ctnnb1 in mouse liver.DepositorInsertsgCTNNB1 Nicking (Ctnnb1 Mouse)
ExpressionMammalianAvailable SinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro
Plasmid#216247PurposeExpress OsTIR1.F74A mutant with mCherry tag for AID2DepositorInsertOsTIR1.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianPromoterEF1aAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only