We narrowed to 6,131 results for: pcDNA 3.1

Showing: 5801 - 5820 of 6131 results

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FRT1-Mb2YFP-2A (IG153)

Plasmid

#99619

Purpose

To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT2-Mb2Tomato-2A (IG154)

Plasmid

#99620

Purpose

To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT3-HA-H2B-Cerulean-2A (IG158))

Plasmid

#99624

Purpose

To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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p16-GFP-CYRI-A

Plasmid

#247175

Purpose

Expresses recombinant fluorescent CYRI-A (Fam49A) protein in mammalian cells, specifically mouse Cyri-A with an internal GFP after proline 16, preserving the proposed N-terminal myristoylation site.

Depositor

Laura Machesky

Article

Le et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Cyri-A (Cyria Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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p16-mCherry-CYRI-A

Plasmid

#247375

Purpose

Expresses recombinant fluorescent CYRI-A (Fam49A) protein in mammalian cells, specifically mouse Cyri-A with internal mCherry after proline 16, preserving the proposed N-terminal myristoylation site.

Depositor

Laura Machesky

Article

Le et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Cyri-A (Cyria Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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MOR FRET sensor

Plasmid

#231807

Purpose

FRET-based intramolecular conformation sensor for rat MOR

Depositor

Moritz Bünemann

Article

Kirchhofer et al Commun Biol. 2025 Feb 8;8(1):198. doi: 1

Insert

µ-Opioid Receptor (Oprm1 Rat)

Tags

mTurq2 and sYFP2 (3rd loop)

Expression

Mammalian

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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NOP FRET sensor

Plasmid

#231809

Purpose

FRET-based intramolecular conformation sensor for human nociceptin receptor NOP

Depositor

Moritz Bünemann

Article

Kirchhofer et al Commun Biol. 2025 Feb 8;8(1):198. doi: 1

Insert

Nociceptin Receptor (PNOC Human)

Tags

mTurq2 and sYFP2 (3rd loop)

Expression

Mammalian

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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MOR BRET sensor

Plasmid

#231810

Purpose

BRET-based intramolecular conformation sensor for rat MOR

Depositor

Moritz Bünemann

Article

Kirchhofer et al Commun Biol. 2025 Feb 8;8(1):198. doi: 1

Insert

µ-Opioid Receptor (Oprm1 Rat)

Tags

nLuc and sYFP2 (3rd loop)

Expression

Mammalian

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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AcrVA1_AsLOV2-S103ΔELS

Plasmid

#246049

Purpose

Expression of AcrVA1-AsLOV2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables optogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_AsLOV2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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AcrVA1_cpGR2(GGS5)-S103ΔELS

Plasmid

#246050

Purpose

Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables chemogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_cpGR2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA4_cpGR2(GGS5)-ΔN64_Q65_E66

Plasmid

#246052

Purpose

Expression of AcrIIA4-cpGR2 hybrid in mammalian cells after Bubeck et al Nat Methods 2018; enables chemogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA4_cpGR2-ΔN64/Q65/E66

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔN64/Q65/E66

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHCFnc/V5

Plasmid

#247003

Purpose

Expresses non cleavable HCF-1 with carboxy terminal V5 tag under control of the CMV promoter

Depositor

Thomas Kristie

Article

Vogel et al Proc Natl Acad Sci U S A. 2006 May 2;103

Insert

HCF-1 (HCFC1 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

Glu to Ala change at amino acid positions 1019,10…

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAD120-CHIP-G132N-myc-His

Plasmid

#236053

Purpose

Mammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP-G132N (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

G132N

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAD000-CHIP-K30A-myc-His

Plasmid

#236052

Purpose

Mammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP-K30A (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

K30A

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CHGA-eGFP

Plasmid

#228569

Purpose

Expresses GFP-tagged human Chromogranin A in mammalian cells

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

eGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-T453D

Plasmid

#235209

Purpose

Expresses GRP78 with T453D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Threonine 453 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-R197H

Plasmid

#235208

Purpose

Expresses GRP78 with R197H mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Arginine 197 to Histidine

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-G227D

Plasmid

#235207

Purpose

Expresses GRP78 with G227D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Glycine 227 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-NLS-Mut

Plasmid

#235206

Purpose

Expresses GRP78 with mutated NLS in mammalian cells

Depositor

Amy Shiu Lee

Article

Liu et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Mutate the NLS sequence

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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mCGN-Myc-His

Plasmid

#236510

Purpose

For expression of Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Tags

Myc-His

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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