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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,952 results for: sequence

Showing: 8701 - 8730 of 12952 results

  7. pACEBac1-CoRESTfl

    Plasmid
    #109156
    Purpose
    Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Inui et al Protein Sci. 2017 May;26(5):997-1011. do
    Insert
    human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
    Use
    Tags
    3xFLAG
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available Since
    Jan. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    109156-plasmid-map-sequence-id-232884

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  8. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Use
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

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  9. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  13. pRMC2-tat:msfGFP

    Plasmid
    #194915
    Purpose
    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194915-plasmid-map-sequence-id-384626

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  17. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid
    #207634
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207634-plasmid-map-sequence-id-413133

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  19. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid
    #117274
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
    Depositor
    David Stephens
    Article
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Inserts
    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    Use
    Tags
    mScarlet-i
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV and IRES
    Available Since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    117274-plasmid-map-sequence-id-233028

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  23. pCEP4-BG505.SOSIP-TM

    Plasmid
    #111844
    Purpose
    Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 Env (BG505 SOSIP.664)
    Use
    Tags
    CD5 leader sequence and TM helix from MHC class I
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; has SOSIP mutatio…
    Promoter
    CMV
    Available Since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111844-plasmid-map-sequence-id-210213

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  24. pKE13-ISceI-fabp10a:CreERT2

    Plasmid
    #198242
    Purpose
    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase
    Depositor
    Didier Stainier
    Article
    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.
    Inserts
    Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    198242-plasmid-map-sequence-id-393628

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  29. L-GARS-2

    Plasmid
    #166531
    Purpose
    scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166531-plasmid-map-sequence-id-328315

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Showing: 8701 - 8730 of 12952 results