We narrowed to 26,489 results for: CAL

1. pAc5.1B-EGFP-Dm4E-T-LLFWKtoAAAAA_Z

   Plasmid

   #148156

   Purpose

   Insect Expression of Dm4E-T-LLFWKtoAAAAA

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   Dm4E-T-LLFWKtoAAAAA (4E-T Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non silent mutation L127S, annotated as EST, …

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-lambdaN-HA-DmCup_1-417-DmeIF4G_686-1666_T

   Plasmid

   #147584

   Purpose

   Insect Expression of DmCup_1-417-DmeIF4G_686-1666

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   DmCup_1-417-DmeIF4G_686-1666 (cup Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-Dm4E-T_1-510-Y10AL15A-V5His6_T

   Plasmid

   #147600

   Purpose

   Insect Expression of Dm4E-T_1-510-Y10AL15A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   Dm4E-T_1-510-Y10AL15A (4E-T Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pnYC-NpH-CeeIF4E3_1-215opt_AB

   Plasmid

   #148367

   Purpose

   Bacterial Expression of CeeIF4E3_1-215

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

   Insert

   CeeIF4E3_1-215
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pnYC-NpH-CeeIF4E3_30-215opt_AB

   Plasmid

   #148369

   Purpose

   Bacterial Expression of CeeIF4E3_30-215opt

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

   Insert

   CeeIF4E3_30-215opt
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPO812

   Plasmid

   #195306

   Purpose

   pBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose inducible arapBAD

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOPO646

   Plasmid

   #195307

   Purpose

   pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsD of McCAST
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose inducible arapBAD

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-Rtn2b-R60f-mCherry

   Plasmid

   #186607

   Purpose

   Third generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherry

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 mutant R60f (RTN2 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   c.178insC (R60f)

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Rtn2b-NTD136-271-mNeon

   Plasmid

   #186604

   Purpose

   Encodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-miniSOG-C-Far

    Plasmid

    #193914

    Purpose

    Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GB2890

    Plasmid

    #193133

    Purpose

    A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1dG2b.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GB1839

    Plasmid

    #193103

    Purpose

    GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP gRNA2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-RCC

    Plasmid

    #190635

    Purpose

    Mammalian expression of genetically encoded calcium indicator RCC

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    RCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI

    Plasmid

    #191473

    Purpose

    Negative control for ratiometric comparison between green fluorescent proteins and sensors

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    none
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDRESS-mTq2-antiFRET-P2A2-linker_noSacI

    Plasmid

    #191472

    Purpose

    Negative control for ratiometric comparison between red fluorescent proteins and sensors

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    none
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-WIPI2d (FRRG/AAAA)-TS

    Plasmid

    #190037

    Purpose

    Expression of wipi2d mutant

    Depositor

    James Hurley

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    TEV-2xStrep tag

    Expression

    Mammalian

    Mutation

    223FRRG to AAAA

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mini-talin P229S (headR13DD P229S)

    Plasmid

    #182995

    Purpose

    expression of minitalin P229S in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    Talin 1 (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    P229S

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. talin1-head P229S

    Plasmid

    #182997

    Purpose

    expression of talin1 head P229S in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    talin1 head P229S (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    P229S

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

    Plasmid

    #191176

    Purpose

    Mammalian expression of EK0209 with a second stop codon.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    CMV-TO:mCherry:s70587:EGFP (double-stop)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    additional stop codon

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191178

    Purpose

    Mammalian expression of EK0493 with second stop codon.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:GAPDH.s1:EGFP (double-stop)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    additional stop codon

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)

    Plasmid

    #191158

    Purpose

    Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp long

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1:Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

    Plasmid

    #191166

    Purpose

    Mammalian expression of a EGFP mutant N213D derived from EK0379.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP(N213D)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.637A>G(N213D; CCA>CCG)

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EK0362 SFFV-mCherry-SG(Bdnf.s1L36+54@202)SG-EGFP (FLP-IN)

    Plasmid

    #191153

    Purpose

    Mammalian expression of a 90 bp (36+54) long sensor for murine Bdnf 3' UTR where the trigger sequence is split

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L36+54:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. nPPARg429-gs10-NLS-VPR

    Plasmid

    #188266

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nPPARg429 (PPARG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA-SCcpV

    Plasmid

    #190636

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCcpV

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCcpV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pfa6a mScarlet-FKBP::KANMX

    Plasmid

    #185905

    Purpose

    Plasmid for cloning mScarlet fused to FKBP into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-FKBP
    

    Use

    Tags

    mScarlet fused to FKBP

    Expression

    Yeast

    Mutation

    Promoter

    SP6

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MAC_C_FLT1

    Plasmid

    #187784

    Purpose

    MAC-tagged gene expression of human FLT1

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAC_C_FLT4

    Plasmid

    #187769

    Purpose

    MAC-tagged gene expression of human FLT4

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    FLT4 (FLT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mTln1-F2-pET151

    Plasmid

    #177870

    Purpose

    Expresses mouse Talin 1 F2 domain in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2 domain (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mTln1-F2F3-pET151

    Plasmid

    #177877

    Purpose

    Expresses mouse Talin 1 F2F3 domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 domain (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mVenus-FAK(397)FRB

    Plasmid

    #188660

    Purpose

    mVenus tagged Focal Adhesion Kinase with FRB inserted at Y397

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Insertion of FRB at Tyrosine 397

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET303-ColE1-TR-del1-40

    Plasmid

    #180236

    Purpose

    Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364)

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T and R domains (r…

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET303-ColE1-T-P110A

    Plasmid

    #180237

    Purpose

    Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T domain of ColE1 …

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Cherry-Plekhh1 5 silent mutations

    Plasmid

    #187373

    Purpose

    Expresses human Plekhh1 with 5 silent mutations labelled with Cherry

    Depositor

    Evelyne Coudrier

    Article

    Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

    Insert

    Plekhh1 with 5 silent mutations
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Five silent mutations at the Plekhh1 siRNA site (…

    Promoter

    CMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNCS-Clover4-LUMABS-HIV

    Plasmid

    #183485

    Purpose

    To express the Clover4-LUMABS-HIV sensor for Acquired Immunodeficiency Virus antibody dection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-HIV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFastBac1-Clover4-LUMABS-S1 RBD

    Plasmid

    #183491

    Purpose

    To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-S1 RBD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNCS-Clover4-LUMABS-EBNA1

    Plasmid

    #183488

    Purpose

    To express the Clover4-LUMABS-EBNA1 sensor for Epstein-barr Virus antibody dection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-EBNA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNCS-Clover4-LUMABS-VCA

    Plasmid

    #183489

    Purpose

    To express the Clover4-LUMABS-VCA sensor for Epstein-barr Virus antibody dection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-VCA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Use

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    none

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT2h70CRET(#325)

    Plasmid

    #184060

    Purpose

    cyclofen-inducible CRE activation in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

    Insert

    CRE-ERT2
    

    Use

    Zebrafish transient transgenesis

    Tags

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Hsp70-4

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge