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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,987 results for: nes

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Showing: 5801 - 5820 of 15987 results

  1. KIFC1

    Plasmid
    #25253
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIFC1:G361-C715 (KIFC1 Human)
    Use
    Baculovirus expression
    Tags
    His
    Mutation
    Contains amino acids G361-C715
    Promoter
    polyhedrin
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  5. mRaspberry-C1

    Plasmid
    #54659
    Purpose
    Localization: C1 Cloning Vector, Excitation: 598, Emission: 625
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mRaspberry
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pGCGFP-puro

    Plasmid
    #31266
    Depositor
    Andrew Pierce
    Insert
    GCGFP
    Expression
    Mammalian
    Available Since
    Sept. 19, 2011
    Availability
    Academic Institutions and Nonprofits only
    31266-plasmid-map-sequence-id-14329

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  12. pMK-Cas9-gate

    Plasmid
    #113743
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR and Gateway Cloning
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113743-plasmid-map-sequence-id-220965

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  13. HeFm2SpCas9

    Plasmid
    #92111
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    “Highly enhanced Fidelity” mut2 SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92111-plasmid-map-sequence-id-197616

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Showing: 5801 - 5820 of 15987 results