We narrowed to 10,611 results for: t7

1. pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

   Plasmid

   #136627

   Purpose

   Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-LAMA-R59
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SENP2M497A

   Plasmid

   #61088

   Purpose

   cleave the SUMO off when use PETSUMO vector for express fusion protein

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   SENP2M497A catalytic domain (SENP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   M497A; Catalytic domain is residues 364-589

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. HSA2_His

   Plasmid

   #202764

   Purpose

   human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA2
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Lwa_Cas13a_WT

   Plasmid

   #91865

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LwaCas13a
   

   Use

   CRISPR

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWUR702

   Plasmid

   #53079

   Purpose

   Inducible TtAgo expression

   Depositor

   John van der Oost

   Article

   Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

   Insert

   TtAgo
   

   Tags

   Strep-II tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAL2289

   Plasmid

   #36724

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FANCG-TALEN_Right (Fancg Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pR0306 CcaCas13b His6-TwinStrep-SUMO

   Plasmid

   #182687

   Purpose

   Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   CcaCas13b
   

   Tags

   SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-vhhGFP4-hIgG1-Fc

   Plasmid

   #105576

   Purpose

   T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
   

   Use

   In vitro transcription

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDC216

   Plasmid

   #115791

   Purpose

   Expresses rat Syntaxin 1

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   Syntaxin1 (Stx1a Rat)
   

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   C145S S193C C271S C272S

   Promoter

   T7

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1B-GFP1-10

    Plasmid

    #193867

    Purpose

    E. coli expression vector for Hisx6 tagged GFP1-10.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    sfGFP1-10
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpyDock

    Plasmid

    #124618

    Purpose

    Expresses SpyDock to bind SpyTag non-covalently for affinity purification

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyDock
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1_mNeongreen

    Plasmid

    #182413

    Purpose

    Bacterial expression of mNeongreen

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNP1830

    Plasmid

    #222728

    Purpose

    pNP561 - T5 Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. modRNAc0-Cas9-2A-GFP

    Plasmid

    #170181

    Purpose

    IVT template plasmid for making modified Cas9GFP RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9-2A-GFP
    

    Use

    Ivt template plasmid

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET16b-Trx

    Plasmid

    #36944

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. CD8a

    Plasmid

    #86050

    Purpose

    A mammalian expression plasmid encoding CD8a without tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7C-BAG2

    Plasmid

    #177654

    Purpose

    Recombinant protein synthesis of human BAG2

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    BAG2 (BAG2 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FKBP-Venus-173-C

    Plasmid

    #87617

    Purpose

    FKBP-tagged C-terminal Venus (YFP) fragment for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    FKBP-Venus173-C
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-hDcp2

    Plasmid

    #72214

    Purpose

    bacterial expression of His-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only