We narrowed to 10,102 results for: otos

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pCDNAIIIB rac1 QL

Plasmid

#61633

Purpose

mammalian expression of rac1 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-FLEX-Mac-GFP

Plasmid

#58852

Purpose

AAV-mediated expression of Mac-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR AE Clover

Plasmid

#90240

Purpose

Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-SFPQY527A

Plasmid

#166961

Purpose

Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Tags

FLAG

Expression

Mammalian

Mutation

SFPQ-Y527A

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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Neurod1-Atoh7HA3

Plasmid

#44438

Depositor

William Klein

Article

Mao et al Development. 2013 Feb;140(3):541-51. doi

Insert

Atoh7-3xHA knock-in (Atoh7 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Neurod1 left arm, Neurod1 right arm, and loxC2-Re…

Expression

Mammalian

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

Plasmid

#124660

Purpose

reporter protein which emits bright blue fluorescence

Depositor

Yutaka Kuroda

Article

Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

Insert

Gaussia luciferase-C9D-TGinsert

Tags

IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

Expression

Bacterial

Mutation

changed glutamic acid 100 to alanine, and glycine…

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT2m

Plasmid

#208722

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2m

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-N-terminus(1-111)

Plasmid

#64671

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only N-terminus amino acids 1 to 111

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pBabepuro3-p18

Plasmid

#24935

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p18 (CDKN2C Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

Plasmid

#107727

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD mApple

Plasmid

#91772

Purpose

Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

monomeric Apple

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

R97K, E152S, A180S, V207T compared to mApple (FP …

Promoter

araBAD

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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p10xUAS-IVS- Syn21-Voltron-p10

Plasmid

#119041

Purpose

UAS-driven expresion of Voltron in Drosophila

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Insect

Promoter

hsp70

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-C-terminus

Plasmid

#64673

Purpose

expresses only C terminus of RNF8, amino acids 402-485

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only C-terminus amino acids 402 to 485

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pCI-neo-myc-LC3(deltaC22)

Plasmid

#45448

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pFIN-XOPS-tdTOM-MOPS-GFP

Plasmid

#44347

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

XOPS-tdTOM
MOPS-GFP

Use

Lentiviral

Promoter

Xenopus Opsin (XOPS) (pos 3131-4495) and mouse op…

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-mCherry

Plasmid

#72898

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E4_47-300 (pBS0847)

Plasmid

#185279

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_47-300

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Integrin-Beta1-N-18

Plasmid

#55423

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only