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pCAGGS-I-SceI-Trex2

Plasmid

#44024

Depositor

Jeremy Stark

Article

Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

Insert

I-SceI fused to mTREX2

Tags

NLS-HA

Expression

Mammalian

Promoter

pCAGGS

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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Cas9 Null

Plasmid

#198727

Purpose

Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-PLIN3

Plasmid

#222420

Purpose

To express a lipid droplet "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

PLIN3 (PLIN3 Human)

Tags

FRB-mCherry

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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tdTomato-LacI

Plasmid

#183921

Purpose

Lac repressor labeled with tdTomato; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdTomato-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMRXIP SECFP-Stx17TM

Plasmid

#86777

Purpose

Expresses transmembrane domains of syntaxin17 tagged with SECFP in mammalian cells

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

transmembrane domains of human Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

SECFP

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV

Plasmid

#235534

Purpose

To express GFP

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagRFP657-2A

Plasmid

#235537

Purpose

To express tagRFP657-2A

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCB6-HFE-EGFP

Plasmid

#12104

Depositor

Pamela Bjorkman

Article

Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-

Insert

HFE (HFE Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
NLSx3-CRY2PHR-p65

Plasmid

#64124

Purpose

Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

CRY2PHR-p65

Tags

His, NLSx3, and V5

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pPRIME-dsRed-shscramble

Plasmid

#71384

Purpose

Expresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cells

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

CMV promoter-dsRed-mir30-shscramble

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV enhancer/promoter

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLCRISPR-CMV

Plasmid

#102609

Purpose

Control lentivector expressing Cas9 and gRNA scaffold

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhab-3'UTR

Plasmid

#61782

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR

Plasmid

#61776

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Fhod1-3'UTR

Plasmid

#61773

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR

Plasmid

#61783

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

MBP promoter fragment (Mbp Mouse)

Expression

Mammalian

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mCit-IRES-Neo

Plasmid

#155230

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mCitrine sensor

Use

Lentiviral

Tags

mCitrine

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pLG017 (AVAST type 1)

Plasmid

#157895

Purpose

Expresses the AVAST type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 1 (STAND ATPase with N-terminal serine protease)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM60-Auf1

Plasmid

#38242

Purpose

3rd generation lentiviral vector

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

AUF1 (HNRNPD Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pQCXIN-EF1a-mNeonGreen-P2A-Cas9

Plasmid

#189807

Purpose

Retroviral delivery of Cas9

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Cas9

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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