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pNTI935

Plasmid

#216638

Purpose

cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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mRuby2-Rab5a-7

Plasmid

#55911

Purpose

Localization: Ras GTPase, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Rab5a

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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TBP6.7 non-targeting gRNA lambda phage

Plasmid

#132547

Purpose

expresses gRNA for TBP-based CIRTS

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

gRNA TBP-based CIRTS

Expression

Mammalian

Promoter

hU6 promoter

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS2164_Nme2-ABE8e-i7

Plasmid

#201517

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i7

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i7

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-cyto

Plasmid

#113914

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Plasmid

#132544

Purpose

Expresses CIRTS-6 (bdefensin 3-TBP6.7-YTHDF2(1-200)) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NAD-Snifit

Plasmid

#113658

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB2

Plasmid

#222532

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB2 (HMGB2 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Vinculin-N-21

Plasmid

#55160

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBJ1

Plasmid

#167130

Purpose

A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Frr promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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FusionRed-Lifeact-7

Plasmid

#54778

Purpose

Localization: Actin, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Lifeact-FusionRed

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pEBTet_NAD-Snifit-nucl

Plasmid

#113915

Purpose

Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-HaloTag7_T2A_mEGFP

Plasmid

#187072

Purpose

Mitochondria surface expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-HaloTag7

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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mEos2-ER-5

Plasmid

#57373

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

ER (CALR Human)

Tags

mEos2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pfli1

Plasmid

#22413

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

fli1 (fli1 Zebrafish)

Use

Riboprobe

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_mEGFP

Plasmid

#187073

Purpose

Mitochondria surface expression of dHaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
Dendra2-Golgi-7

Plasmid

#57722

Purpose

Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Golgi (B4GALT1 Human)

Tags

Dendra2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEJS2191_Nme2-evoFERNY-nt

Plasmid

#201527

Purpose

Mammalian expression of N-terminally fused Nme2-evoFERNY-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-evoFERNY-nt

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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