161,873 results

1. PB-CMV-MCS-Puro-EGFP

   Plasmid

   #219795

   Purpose

   Mammalian expression plasmids using piggyBac Transposon System

   Depositor

   Keiichi Nakayama

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRK5-FLAG-Bag6ΔUBL

   Plasmid

   #61837

   Purpose

   express N-terminally FLAG tagged human Bag6 without UBL domain

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   Bag6 (BAG6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-HA-Flag-natT1R2

   Plasmid

   #113944

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18T-mini-Tn7T-hph-dCas9

   Plasmid

   #218371

   Purpose

   Expression of dCas9 for CRISPR-interference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PtetA

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWH1266-Apra-sgRNA

   Plasmid

   #218372

   Purpose

   sgRNA expression plasmid for CRISPR-intereference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   sgRNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

   Plasmid

   #234952

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1-Tspan4

   Plasmid

   #210323

   Purpose

   Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

   Depositor

   Li Yu

   Article

   Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

   Insert

   Tetraspanin 4 (Tspan4 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. VPS13C^mClover3

   Plasmid

   #118760

   Purpose

   VPS13C internally tagged with mClover3 at residue 1914.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13C (VPS13C Human)
   

   Use

   Tags

   mClover3 inserted at position aa 1914

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-mCherry-Cre-blast

   Plasmid

   #179390

   Purpose

   Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

   Insert

   mCherry (P2A) Cre recombinase
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCre

    Plasmid

    #236212

    Purpose

    Arabinose-inducible Cre recombinase

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    Cre recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. PLKO.1-Scrambled

    Plasmid

    #136035

    Purpose

    Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    None (Scrambled)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMV RasV12 Neo (w108-1)

    Plasmid

    #22259

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V mutant

    Promoter

    Available since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

    Plasmid

    #29722

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5M-ADAM10

    Plasmid

    #31717

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF0694

    Plasmid

    #141664

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP hsp70 K71E

    Plasmid

    #15216

    Depositor

    Lois Greene

    Article

    Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

    Insert

    hsp70 K71E (HSPA1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K71E, ATPase-deficient mutant.

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-syn-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234438

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0985

    Plasmid

    #142249

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Plasmid

    #180475

    Purpose

    Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Zebrafish ubb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBbsr-JNK KTR-mCherry

    Plasmid

    #115493

    Purpose

    Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

    Insert

    JNK KTR-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry G229L, mCherry Y298 deleted

    Promoter

    CAG

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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