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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,184 results for: Pol;

Showing: 8791 - 8820 of 9184 results

  4. MmEos3.2

    Plasmid
    #203754
    Purpose
    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    Src15 n-term (SRC Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203754-plasmid-map-sequence-id-403344

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  6. pKE13-ISceI-fabp10a:CreERT2

    Plasmid
    #198242
    Purpose
    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase
    Depositor
    Didier Stainier
    Article
    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.
    Inserts
    Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    198242-plasmid-map-sequence-id-393628

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  11. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid
    #89154
    Purpose
    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli
    Depositor
    Frédéric Allain
    Article
    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n
    Insert
    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    Tags
    Intein and chitin binding domain
    Expression
    Bacterial
    Mutation
    mutations: Cys 250 to Ser, Cys 251 to Ser
    Promoter
    T7
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89154-plasmid-map-sequence-id-175723

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  12. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid
    #52890
    Purpose
    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.
    Depositor
    Iswar Hariharan
    Article
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Inserts
    LexGAD
    Gal4 (GAL4 Budding Yeast)
    Expression
    Insect
    Available Since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    52890-plasmid-map-sequence-id-211914

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  14. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Stefan Tenzer
    Article
    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102
    Insert
    ATI QconCAT
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163957-plasmid-map-sequence-id-321834

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  15. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do
    Insert
    Full-length engineered CD64, derived from human CD64
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    109191-plasmid-map-sequence-id-233794

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  18. pLKO.1-shGBP1.1.mKO2

    Plasmid
    #85209
    Purpose
    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    GBP1 (guanylate binding protein 1) (GBP1 Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available Since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85209-plasmid-map-sequence-id-169780

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  19. tRNA-gRNA position [D1_n-1] (GB1208)

    Plasmid
    #75409
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [D1_n-1]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75409-plasmid-map-sequence-id-211946

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  20. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Expression
    Bacterial
    Available Since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. MKC131_CCR5(SFFV-synEPOR-2A-YFP)

    Plasmid
    #232412
    Purpose
    AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Expression
    Mammalian
    Available Since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232412-plasmid-map-sequence-id-465038

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Showing: 8791 - 8820 of 9184 results