We narrowed to 23,735 results for: fus
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pAAV hGRN2
Plasmid#213683PurposeExpresses human granulin-2 with an N-terminal twin-Strep-FLAG tagDepositorInserthuman Granulin-2
UseAAVTagstwin-Strep-FLAGPromotercytomegalovirus enhancer/chicken β-actin promoterAvailable SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV GFP
Plasmid#213685PurposeExpresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tagDepositorInsertTwin-Strep GFP
UseAAVTagsTwin-StrepPromotercytomegalovirus enhancer/chicken β-actin promoterAvailable SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46-His (SB250)
Plasmid#227599PurposeInducible expression of His-tagged SLC25A46 in Pichia pastorisDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)
Plasmid#227614PurposeOverexpression of SLC25A46 in mammalian cell linesDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-Tudor-R rich
Plasmid#225478PurposeExpress mEGFP-GAPDH-Tudor-R fusionDepositorInsertGAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)
TagsmEGFPExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS9
Plasmid#218717PurposeExpresses N-terminally EGFP-tagged BBS9 in mammalian cellsDepositorAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.Z-GS3
Plasmid#204758PurposeExpression of Cas9 and human H2A.ZDepositorInsertH2AZ1 (H2AZ1 Human)
ExpressionMammalianAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403) L361C
Plasmid#213622PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403) L361CDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-cPLA2 C2 domain (Y96C)
Plasmid#213633PurposeBacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)DepositorInsertcPLA2 C2 domain (Y96C) (PLA2G4A Human)
TagsSUMOExpressionBacterialMutationY96C, C139A, C141SAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb wnt3 promoter-Fzd1mApple
Plasmid#203795PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesisDepositorAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-Villin
Plasmid#200301PurposeBacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35DepositorInsertbeta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialPromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-mini
Plasmid#196338PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1D
Plasmid#196686PurposeRep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationPTQGTFR insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.1
Plasmid#196687PurposeRep/Cap plasmid for the production of M.Fas.1, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationTDALTTK insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1B
Plasmid#196684PurposeRep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationPSQGTLR insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1A
Plasmid#196683PurposeRep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationPTQGTVR insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.4
Plasmid#196690PurposeRep/Cap plasmid for the production of M.Fas.4, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationVVSDYTV insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.3
Plasmid#196689PurposeRep/Cap plasmid for the production of M.Fas.3, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRVDPSGL insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.2
Plasmid#196688PurposeRep/Cap plasmid for the production of M.Fas.2, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationSTIPTMK insert between amino acids 588 and 589 of…Promoterp41Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-mNG
Plasmid#195160Purposemammalian expression of siRNA-resistant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Stim1NTD-ABHD16Acyto-mNG
Plasmid#195158Purposemammalian expression of ER localized ABHD16A tagged with mNeonGreenDepositorInsertStim1NTD (STIM1 Human)
TagsABHD16A cytoplasmic domain and mNeonGreenExpressionMammalianMutationNonePromoterCMVAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
DsRed -7e
Plasmid#191765PurposeExpression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7eDepositorInsertDsRed
TagsN terminal fusion of negatively charged polypetod…ExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-2xTS
Plasmid#162779PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP- Ura
Plasmid#177732PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA markerDepositorInsertFlucDM
TagsHA, HDEL, KAR2 Signal Sequence, and eGFPExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only
