We narrowed to 22,836 results for: Sis

1. pRWJB006

   Plasmid

   #160113

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   35S

   Available Since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRWJB004

   Plasmid

   #160112

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   UBQ10

   Available Since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK528

   Plasmid

   #155388

   Purpose

   dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-mEGFP-HO-G

   Plasmid

   #164163

   Purpose

   Expresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminal

   Depositor

   Isei Tanida

   Article

   Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

   Insert

   mEGFP A236K
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p8115 LentiCRISPR v2 sgPTPN14-3

   Plasmid

   #163314

   Purpose

   Expresses Cas9 and a human PTPN14-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   sgPTPN14-3
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw

   Plasmid

   #162473

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreAB
   

   Use

   Cre/Lox

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw

   Plasmid

   #162478

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreAB
   

   Use

   Cre/Lox

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw

   Plasmid

   #162476

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreB
   

   Use

   Cre/Lox

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw

   Plasmid

   #162475

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreA
   

   Use

   Cre/Lox

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw

    Plasmid

    #162472

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreC
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw

    Plasmid

    #162470

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreA
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw

    Plasmid

    #162477

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreC
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw

    Plasmid

    #162471

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreB
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid

    #160890

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-S1H
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    rbcS-SH1: T7, chaperones: T13

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid

    #160887

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T4a
    

    Expression

    Bacterial

    Promoter

    rbcS-T4a: T7, chaperones: T10

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE669

    Plasmid

    #153235

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDUAL-Pnmt1-Ost4-GFP

    Plasmid

    #154368

    Purpose

    Yeast expression of Ost4

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Ost4 (ost4 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGE672

    Plasmid

    #153238

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15-His-∆NGlpG(Y205F)

    Plasmid

    #155132

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y205F, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGE473

    Plasmid

    #153264

    Purpose

    M1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDGE500

    Plasmid

    #153273

    Purpose

    M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDGE502

    Plasmid

    #153275

    Purpose

    M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-Myosin7B-sgRNA

    Plasmid

    #154861

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    MYO7B sgRNA (MYO7B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMAL-C2X dAmp/Kana+-2MT

    Plasmid

    #154353

    Purpose

    Bacterial expression of 2MT

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    2MT
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDUAL-P41nmt1-Sad1-GFP

    Plasmid

    #154366

    Purpose

    Yeast expression of Sad1

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Sad1 (sad1 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDUAL-P41nmt1-Nup124-GFP

    Plasmid

    #154364

    Purpose

    Yeast expression of Nup124

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Nup124 (nup124 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDUAL-Pnmt1-Ost4-GFP-MTalpha

    Plasmid

    #154370

    Purpose

    Yeast expression of Ost4

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Ost4 (ost4 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDUAL-P41nmt1-Sad1-GFP-MTn

    Plasmid

    #154367

    Purpose

    Yeast expression of Sad1

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Sad1 (sad1 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSFDuet1-tglA(C51)HIB

    Plasmid

    #134675

    Purpose

    Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglA(C51)
    TglH
    TglI
    TglB
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    insertion of cysteine 51

    Promoter

    T7

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFloat.assemble(AB).LC_v1

    Plasmid

    #133083

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Expression

    Bacterial

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFloat.assemble(AB).HC_v1

    Plasmid

    #133084

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Expression

    Bacterial

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCB6-Occludin

    Plasmid

    #136637

    Purpose

    Expression of Occludin in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    Occludin (OCLN Chicken)
    

    Expression

    Mammalian

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCB6-HAOccludin

    Plasmid

    #136638

    Purpose

    Expression of HAOccludin in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    HAOccludin (OCLN Chicken)
    

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCB6-OccludinCT3

    Plasmid

    #136639

    Purpose

    Expression of OccludinCT3 in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    OccludinCT3 (OCLN Chicken)
    

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal cytoplasid domain

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCB6-HAOccludinCT3

    Plasmid

    #136640

    Purpose

    Expression of HAOccludinCT3 in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    HAOccludinCT3 (OCLN Chicken)
    

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal cytoplasid domain

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pY71-tet.I.-1

    Plasmid

    #133539

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pY71-tet.I.25

    Plasmid

    #133538

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pY71-tet.I.20

    Plasmid

    #133537

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pY71-tet.I.14

    Plasmid

    #133535

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pY71-tet.I.13

    Plasmid

    #133534

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge