We narrowed to 23,941 results for: nes

1. pDY390

   Plasmid

   #182963

   Purpose

   Amp-resistant, low copy (p15A ori), E. coli ptsI transcriptionally fused with GFP.

   Depositor

   Shelley Copley

   Article

   Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

   Insert

   ptsI
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHyg-CUbo(K63R)-9myc

   Plasmid

   #212788

   Purpose

   C-terminal PCR-based tagging with CUbo(K63R)-9myc in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHyg-CUbo-AID*-GFP

   Plasmid

   #212770

   Purpose

   C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUra3-pCUP1-CUbo(K63R)

   Plasmid

   #212764

   Purpose

   N-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUra3-pCUP1-CUbo(K48R)

   Plasmid

   #212763

   Purpose

   N-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUra3-pCUP1-CUbo

   Plasmid

   #212762

   Purpose

   N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUra3-pCUP1-His7-NUbo

   Plasmid

   #212761

   Purpose

   N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUra3-pCUP1-NUbo

   Plasmid

   #212760

   Purpose

   N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAC150-FAM134C-GFP

   Plasmid

   #201932

   Purpose

   PiggyBac Vector expressing FAM134C-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   FAM134C (RETREG3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC150-TEX264ΔLIR-GFP

    Plasmid

    #201930

    Purpose

    PiggyBac Vector expressing TEX264ΔLIR-GFP

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F273A

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET24a-His6-CUbo-MCS

    Plasmid

    #212725

    Purpose

    Bacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET24a-His7-NUbo-MCS

    Plasmid

    #212724

    Purpose

    Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24a-NUbo-MCS-His6

    Plasmid

    #212723

    Purpose

    Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET24a-NUbo-E3(63)-His6

    Plasmid

    #212717

    Purpose

    Bacterial expression of NUbo-E3(63)-His6

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Tags

    6xHis and NUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET24a-NUbo-E3(48)-His6

    Plasmid

    #212714

    Purpose

    Bacterial expression of NUbo-E3(48)-His6

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Tags

    6xHis and NUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1-shCtnnd2.1-GFP

    Plasmid

    #209095

    Purpose

    GFP expressing shRNA targeting Ctnnd2 N-terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCtnnd2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-scrCtnnd2.1-mCherry-CAAX

    Plasmid

    #209108

    Purpose

    Membrane tethered mCherry scramble of shRNA targeting Ctnnd2 N-terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCtnnd2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-shCtnnd2.1-mCherry-CAAX

    Plasmid

    #209107

    Purpose

    Membrane tethered mCherry shRNA targeting Ctnnd2 N-terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCtnnd2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-shCdh20.1 GFP

    Plasmid

    #209105

    Purpose

    GFP expressing shRNA targeting Cdh20

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCdh20.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-shCdh10.1 GFP

    Plasmid

    #209101

    Purpose

    GFP expressing shRNA targeting Cdh10

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCdh10.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLKO.1-shCtnnd2.2-GFP

    Plasmid

    #209097

    Purpose

    GFP expressing shRNA targeting Ctnnd2 3' UTR

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCtnnd2.2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO.1-scrCtnnd2.1-GFP

    Plasmid

    #209096

    Purpose

    GFP expressing scramble of shRNA targeting Ctnnd2 N terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCtnnd2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid

    #202586

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid

    #202588

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, changed O…

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Modular BE4max for GG insertion

    Plasmid

    #198891

    Purpose

    Easily insert deaminase of choice into BE4max using Golden Gate cloning

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    CRISPR associated protein 9 (cas9 Streptococcus pyogenes)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Dox-SOScat

    Plasmid

    #207329

    Purpose

    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

    Depositor

    Jared Toettcher

    Article

    Underhill et al Development. 2023 Aug 15;150(16):dev2016

    Insert

    son of sevenless homolog 2 variant, catalytic domain
    

    Use

    Tags

    myristoylation tag - BFP

    Expression

    Bacterial

    Mutation

    range: 593 to 1078

    Promoter

    Tet Response Element

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-C1-hEFA6C

    Plasmid

    #206414

    Purpose

    Mammalian expression of hEFA6C

    Depositor

    Hye-Won Shin, Kazuhisa Nakayama

    Article

    Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

    Insert

    EFA6C (PSD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GW1-mCherryEA

    Plasmid

    #207867

    Purpose

    Mammalian expression (cytosol) of the mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

    Depositor

    Mathew Tantama

    Article

    Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

    Insert

    mCherryEA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

    Plasmid

    #193050

    Purpose

    Encodes guide RNA expression targeting PX740 landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    Guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHWBF09exp

    Plasmid

    #130833

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP1
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. MLKP2del4

    Plasmid

    #130837

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP2
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of terminal four amino acid residues

    Promoter

    CaMV 35S

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. MLKP1delTM

    Plasmid

    #130841

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP1
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of transmembrane domain

    Promoter

    CaMV 35S

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. MLKG1delKASH

    Plasmid

    #130842

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKG1
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of KASH domain

    Promoter

    CaMV 35S

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. MLKP2del4ec

    Plasmid

    #130843

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP2
    

    Use

    Unspecified

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Deletion of terminal four amino acid residues

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. MLKP1del4ec

    Plasmid

    #130844

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP1
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Deletion of terminal four amino acid residues

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. MLKP1delKASHec

    Plasmid

    #130845

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP1
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Deletion of KASH domain, addition of three alanin…

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. MLKG1delKASHec

    Plasmid

    #130846

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKG1
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Deletion of KASH domain

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pHWBF07EC

    Plasmid

    #130828

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP2
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pHWBF09EC

    Plasmid

    #130829

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP1
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. ECPK1D

    Plasmid

    #130830

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKG1
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge