We narrowed to 11,691 results for: NSI

1. Ash1

   Plasmid

   #169711

   Purpose

   Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)

   Depositor

   Tanja Mittag

   Article

   Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

   Insert

   Ash1 (ASH1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Truncation including encoding amino acids 420-500

   Promoter

   T7

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-TAB-mOvol1

   Plasmid

   #120360

   Purpose

   piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   Ovol1 (Ovol1 Mouse)
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   tet-on and CBh

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-GFAP104-ChromeT-mCherry

   Plasmid

   #123126

   Purpose

   AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeT-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

   Promoter

   GFAP104

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psiCHECK2 3'UTR GAC

   Plasmid

   #110401

   Purpose

   Luminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19 KGA HA5'-mKO2-3'

   Plasmid

   #110406

   Purpose

   Homology donor for glutaminase KGA isoform knock-in, mKO2

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK528

   Plasmid

   #155388

   Purpose

   dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

   Plasmid

   #159961

   Purpose

   optoNES construct with modified mTurquoise2-LOV2 RET

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

   Plasmid

   #159965

   Purpose

   optoNES construct with modified mTurquoise2-LOV2 RET

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   CMV-IE

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

   Plasmid

   #159964

   Purpose

   optoNES construct with modified mTurquoise2-LOV2 RET

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   CMV-IE

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159958

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159957

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159960

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159959

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159963

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C335

    Plasmid

    #141125

    Purpose

    Expresses chimeric repressor (RbsR-L) under Cin promoter

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Inserts

    LLhR
    AiiA (AQ980_RS12585 Synthetic)
    

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Chimera: DNA binding domain of LacI and ligand bi…

    Promoter

    engineered cin promoter (responds to C14-HSL) and…

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

    Plasmid

    #140223

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
    

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pY71-tet.I.-1

    Plasmid

    #133539

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY71-tet.I.25

    Plasmid

    #133538

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pY71-tet.I.20

    Plasmid

    #133537

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY71-tet.I.14

    Plasmid

    #133535

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge