164,765 results

1. pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]

   Plasmid

   #78174

   Purpose

   AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   human synapsin

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTECH-chPylRS(IPYE)

   Plasmid

   #99222

   Purpose

   Expression plasmid encoding chPylRS(IPYE) and pylT

   Depositor

   Dieter Söll

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp chPyl(IPYE)RS; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available Since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF0275

   Plasmid

   #142582

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF3 (TCF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgSmarca4#1/Cre

   Plasmid

   #173619

   Purpose

   Expresses a Smarca4-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Smarca4 (Smarca4 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

   Plasmid

   #172065

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pRK7-HA-S6K1-F5A-E389-deltaCT

   Plasmid

   #8990

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F5A, T389E, stop codon at amino acid 402

   Promoter

   Available Since

   Sept. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

7. wt dynamin 2 pEGFP

   Plasmid

   #34686

   Depositor

   Sandra Schmid

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D406G, V664A, H692R and Q758R

   Promoter

   CMV

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn5-162

   Plasmid

   #118543

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p426 103Q GAL

   Plasmid

   #1188

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    dSV40 Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2R3a-TagRFP-OcsT

    Plasmid

    #71270

    Purpose

    Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    TagRFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_tre_Cas9

    Plasmid

    #126029

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible Cas9

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    humanized S. pyrogenes Cas9
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha and TRE

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIB-HIS-TEV-scCAK1

    Plasmid

    #177014

    Purpose

    Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBZS-M.CviQIX (NDel15)

    Plasmid

    #198357

    Purpose

    Express M.CviQIX (NDel15) in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviQIX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGKp-GFP-YAP (5SA)

    Plasmid

    #174174

    Purpose

    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Five LATS phosphorylation sites (S61, S109, S127,…

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. MtPT4-p3

    Plasmid

    #160003

    Purpose

    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Inserts

    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBbS6c-RFP

    Plasmid

    #35293

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6-sgfiller-eCas9-T2A-BlastR

    Plasmid

    #172437

    Purpose

    Expresses eCas9, BlastR and sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    eCas9
    BlastR
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Sport6-CD9-pHluorin

    Plasmid

    #130905

    Purpose

    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHluorin (CD9 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    phluorin inserted between Asn-50 and Asn-51 in ex…

    Promoter

    CMV

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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