We narrowed to 31,068 results for: LIS;

1. mouse b8 full length

   Plasmid

   #217817

   Purpose

   Full length mouse integrin b8 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGB8 (Itgb8 Mouse)
   

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

   Plasmid

   #177265

   Purpose

   STREAMING-tag cassette

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag cassette
   

   Use

   Unspecified

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP680

   Plasmid

   #65772

   Purpose

   Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E, R1335Q, and T1337R mutations in Cas9

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hygro-iNGN3

   Plasmid

   #75340

   Purpose

   doxycycline inducible NGN3 gene over-expression

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

   Insert

   NGN3 (NEUROG3 Human)
   

   Expression

   Mammalian

   Promoter

   TRE-tight

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. hKv2.1-HA

   Plasmid

   #111525

   Purpose

   Expresses human WT KCNB1 in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

   Insert

   human Kv2.1 + HA (KCNB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-ATN1

   Plasmid

   #16179

   Depositor

   Huda Zoghbi

   Article

   Lim et al Cell. 2006 May 19. 125(4):801-14.

   Insert

   Atrophin 1 (ATN1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

   Plasmid

   #74513

   Purpose

   AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

   Depositor

   Guoping Feng

   Article

   Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

   Insert

   EGFP-p2A-EGFP-f
   

   Use

   AAV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDA095

    Plasmid

    #131391

    Purpose

    generation of Tet-On RPE

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH231-Bx-GFP-C31

    Plasmid

    #115149

    Purpose

    Empty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing pads

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Safe Harbor Homology Sequences
    

    Expression

    Mammalian

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDule2-Tet2.0

    Plasmid

    #85497

    Purpose

    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

    Insert

    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32G L65Q F108S Q109D D158S L162N

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PADH1-TIR1-9myc-URA3

    Plasmid

    #207016

    Purpose

    Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    TIR1
    

    Tags

    9xMyc

    Expression

    Yeast

    Promoter

    pADH1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR223-EBNA3C

    Plasmid

    #198576

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA3C (EBNA-3B/EBNA-3C Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. plexm-human LRRC33

    Plasmid

    #111600

    Purpose

    Express human LRRC33 in 393T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    LRRC33 (NRROS Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB[U6-3 wex2 PUb-DsRed]

    Plasmid

    #169010

    Purpose

    For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    white ex2 sgRNA
    

    Use

    Piggybac

    Expression

    Insect

    Promoter

    Drosophila melanogaster U6:3

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

    Plasmid

    #175563

    Purpose

    pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    GOXL1
    

    Tags

    Citrine

    Expression

    Plant

    Promoter

    RUBY

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse av full length

    Plasmid

    #217809

    Purpose

    Full length mouse integrin av expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGAV (Itgav Mouse)
    

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL11056

    Plasmid

    #68258

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

    Plasmid

    #154002

    Purpose

    Empty SgRNA expression system matching CasRx

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    SgRNA expression system
    

    Use

    CRISPR

    Promoter

    U6, EF1a

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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