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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,428 results

Showing: 5901 - 5920 of 159428 results

  1. pYS17

    Plasmid
    #202083
    Purpose
    Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in
    Depositor
    Daniel Dickinson
    Article
    Shi et al Development. 2023 May 15;150(10):dev2013
    Insert
    NT PuroR-T2A-mNG Minicircle Parent
    Use
    CRISPR
    Tags
    mNeonGreen
    Expression
    Mutation
    Promoter
    None
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202083-plasmid-map-sequence-id-409950

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  2. pCMV-PE7-SB

    Plasmid
    #228869
    Purpose
    PE7 connected to MLH1-SB via 2A sequence
    Depositor
    Sangsu Bae
    Insert
    PE7-2A-MLH1-SB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    228869-plasmid-map-sequence-id-456125

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  5. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  9. TFORF3237

    Plasmid
    #144713
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX10 (SOX10 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144713-plasmid-map-sequence-id-404301

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  10. Myc-ND2 (N72)

    Plasmid
    #22966
    Depositor
    Anirvan Ghosh
    Insert
    NeuroD2 (Neurod2 Mouse)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  14. pcDNA3-ExRai-AktAR2

    Plasmid
    #184047
    Purpose
    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.
    Depositor
    Jin Zhang
    Article
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Insert
    ExRai-AktAR2
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184047-plasmid-map-sequence-id-363388

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  17. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja1 (Gja1 Rat)
    Use
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69007-plasmid-map-sequence-id-121423

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  19. DT480

    Plasmid
    #80413
    Purpose
    Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80413-plasmid-map-sequence-id-151399

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Showing: 5901 - 5920 of 159428 results