We narrowed to 21,470 results for: SPR

Showing: 5901 - 5920 of 21470 results

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PB-EEA-g1-PGK-Puro

Plasmid

#102908

Purpose

PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-guide1-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

Plasmid

#217635

Purpose

AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

Depositor

Martin Kampmann

Article

Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

Insert

mU6-sgRNA, Handle sequence, EF1a-mTagBFP2

Use

AAV

Promoter

EF1alpha and mU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-miR103/107

Plasmid

#21652

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant miR103/107 microRNA binding sites

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pFZ1036

Plasmid

#250676

Purpose

IPT along with RUBY for soybean genome editing

Depositor

Feng Zhang

Article

Alok et al Plant Physiol. 2025 Dec 10:kiaf640. doi:

Insert

IPT, Cas9, RUBY

Use

CRISPR

Expression

Plant

Promoter

CaMV35, AtUbi10

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pFZ1035

Plasmid

#246938

Purpose

GmWUS along with RUBY for soybean genome editing

Depositor

Feng Zhang

Article

Alok et al Plant Physiol. 2025 Dec 10:kiaf640. doi:

Insert

GmWUS, Cas9, RUBY

Use

CRISPR

Expression

Plant

Promoter

NOS, AtUbi10

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-ACME

Plasmid

#186717

Purpose

ACME dual-deaminase CRISPR base editor

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

ACME

Use

CRISPR

Tags

Nucleoplasmin NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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beta2 (px330:2-guideCas9)

Plasmid

#169435

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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Gephyrin (px330:2-guideCas9)

Plasmid

#169432

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pV1010

Plasmid

#111423

Purpose

gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

sgADE-NT2

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2899(X-2 MarkerFree)

Plasmid

#73271

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQAT31

Plasmid

#223403

Purpose

T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

AtUBQ10-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pVP141

Plasmid

#222079

Purpose

Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi

Insert

dCas9-PmCDA1, sfGFP and sacB

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m

Plasmid

#192275

Purpose

Encodes MmdCas12m (D485A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m

Use

CRISPR

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

Plasmid

#167902

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Expression

Bacterial

Promoter

Tet promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pADH143

Plasmid

#90989

Purpose

NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2903(XI-2 MarkerFree)

Plasmid

#73275

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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AP568-3

Plasmid

#70048

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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AP568-2

Plasmid

#70047

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B4

Plasmid

#172845

Purpose

CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mNeonGreen

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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