167,212 results

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pGCS-N2(3xHA)

Plasmid

#85719

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pK19pA-MN

Plasmid

#86973

Purpose

ChIC; genomic mapping of chromatin proteins

Depositor

Ulrich Laemmli

Article

Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

Insert

protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)

Expression

Bacterial

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Flpo

Plasmid

#55634

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV1

Inserts

mCherry
Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLPC-L647RLaminA

Plasmid

#69060

Purpose

encodes a lamin A mutant with Leucine 647 mutated to an Arginin

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

LaminA (LMNA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Lamin A with Leucine 647 mutated to Arginine

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pET-28c S2

Plasmid

#171920

Purpose

Express RNA-based (p)ppGpp sensor

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Insert

RNA-based (p)ppGpp sensor

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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EF05J

Plasmid

#42941

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

Negative control Functionally Dead Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

ORF1 R261A, R262A

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
pTF101.1gw1

Plasmid

#134768

Purpose

A binary plasmid for gateway 1-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialaphos resistance gene (bar) for plant transformation

Depositor

Kan Wang

Article

et al Euphytica (2004) 136: 167.

Type

Empty backbone

Expression

Plant

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pmVen-RecQL4

Plasmid

#211401

Purpose

mammalian expression of human RecQL4 N-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

RecQL4 (RECQL4 Human)

Tags

mVenus

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP

Plasmid

#121907

Purpose

Expresses BirA and eCPX-AP

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
Enhanced Circularly Permuted Outer Membrane Protein X

Tags

6xHis

Expression

Bacterial

Promoter

T7 and pBAD promoter

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA+WT GADD45

Plasmid

#24929

Depositor

Xin Wang

Article

Yang et al J Biol Chem. 2000 Nov 24. 275(47):36892-

Insert

GADD45 (GADD45A Human)

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Expression

Mammalian

Promoter

Pgk

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pIB3

Plasmid

#25452

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCCL-c-MNDU3-X

Plasmid

#81071

Purpose

Empty lentiviral backbone with the MNDU3 promoter

Depositor

Donald Kohn

Article

Logan et al Hum Gene Ther. 2004 Oct;15(10):976-88.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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HP-Cas9hGem-Ade2-LEU2-LINEAR

Plasmid

#174839

Purpose

pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TDH3p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINS-AREL1

Plasmid

#66710

Purpose

Expresses AREL1 HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KIAA0317 (AREL1 )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pCMV6-Neo-ISG15

Plasmid

#80404

Purpose

overexpression of ISG15

Depositor

Isidoro Martinez

Article

Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

Insert

ISG15 (ISG15 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO mScarlet

Plasmid

#131002

Purpose

Double floxed mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

mScarlet

Use

AAV

Promoter

Ef1a

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-dDIO-MCS--4x6T

Plasmid

#196422

Purpose

Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Dre/rox; astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m

Plasmid

#192274

Purpose

Encodes MmCas12m under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12

Use

CRISPR

Expression

Bacterial

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBXNH3

Plasmid

#47067

Purpose

FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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