We narrowed to 19,151 results for: Tat

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pDONR223_PIK3CA_p.E453Q

Plasmid

#82844

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway Cloning

Mutation

E453Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMD19T-psba1-Ppsba2-dCas9-SpR

Plasmid

#73220

Purpose

Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PpsbA2

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-CmCHS-MsCHI

Plasmid

#73398

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-MCS-shCHD5 #1

Plasmid

#68876

Purpose

CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5

Use

AAV and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCaSpeR4 mito roGFP2-Grx1

Plasmid

#65000

Purpose

Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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mChF-AIP(TA)

Plasmid

#61528

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TA mutation)

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP with TA mutation (see comments) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pFPL-Cg

Plasmid

#61652

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Expression

Plant

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only
pEntry Tshld-GFP

Plasmid

#53211

Purpose

pEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld-GFP

Tags

EGFP, Flag, and HA

Promoter

none

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTK25

Plasmid

#37407

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

K82R

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAM29 (wt)

Plasmid

#31143

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM29 (ADAM29 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

WT

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ADAM7 (wt)

Plasmid

#31139

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM7 (ADAM7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wt

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pWZ414-F13

Plasmid

#23056

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
Keratin 8 S73D

Plasmid

#18054

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

S73D

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
-111 hTF m4

Plasmid

#15451

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 Sp1 and EGR-1 sites mutated

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
pSuperRetro-puro-RalB 45i

Plasmid

#11122

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalAB siRNA (RALA, RALB Human)

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pEM09

Plasmid

#135479

Purpose

Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Tags

GFP and hph

Expression

Bacterial and Yeast

Promoter

Spun Hsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLCHKO_PDPR_exon_deletion_1

Plasmid

#155065

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

Plasmid

#233629

Purpose

Expression of bicistronic reporter with mutated TOP motif

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)

Use

Luciferase

Mutation

AGAAGG in TOP motif

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-SPOTlight-U2GCR

Plasmid

#231889

Purpose

Cre-dependent reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Oliver et al eLife 2025 14:RP104457

Insert

DIO-SPOTlight-U2GCR

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK2-CR

Plasmid

#229421

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-CR (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

silent mutation CRISPR resistant

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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