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mTFP1-CathespinB-6

Plasmid

#55467

Purpose

Localization: Lysosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CathespinB

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
CVS-N2c-mCitrine

Plasmid

#172384

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

mCitrine

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAG551-MAP4-greenFAST

Plasmid

#160366

Purpose

Expresses MAP4-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

MAP4-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDM1020

Plasmid

#108977

Purpose

shuttle vector, C-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-ENTR B (708-1)

Plasmid

#17428

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, frame B

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-rsTagRFP

Plasmid

#87620

Purpose

Endoplasmic reticulum (ER)-targeted rsTagRFP.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

ER-rsTagRFP

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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DT042A

Plasmid

#159403

Purpose

Inducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T5-LacO-6xHis-sfGFP

Tags

6xHis

Expression

Bacterial

Promoter

T5 promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWY

Plasmid

#112901

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-H-18

Plasmid

#57422

Purpose

Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin H

Tags

mEos2

Expression

Mammalian

Mutation

Talin Head Domain

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRDA_275

Plasmid

#179091

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-NG

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pdestDD03

Plasmid

#26414

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2 cassette

Expression

Bacterial and Worm

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDM1208

Plasmid

#108985

Purpose

extrachromosomal expression vector, N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector

Tags

mCherry

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tacδ

Plasmid

#81177

Purpose

Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tacδ

Tags

KLGFFKR

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pETM41-EcPPXc

Plasmid

#38329

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
pETM6-M.accABCD

Plasmid

#66865

Purpose

Encodes accA, accB, accC, accD in monocistronic configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-SypRFP

Plasmid

#172381

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

Synaptophysin1-RFP fusion protein

Use

Neurotropic virus

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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GST-U1C

Plasmid

#1840

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

U1C (SNRPC Human)

Tags

GST

Expression

Bacterial

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pISH-Galpha-i2

Plasmid

#15929

Depositor

Peter Mombaerts

Article

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

Galpha-i2 (Gnai2 Mouse)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only
pNUT3

Plasmid

#175744

Purpose

Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3

Depositor

Yubin Zhou

Article

Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

Insert

mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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