We narrowed to 58,550 results for: tra

1. XLone-GvpB

   Plasmid

   #173798

   Purpose

   Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)

   Depositor

   Xiaojun Lian

   Article

   Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

   Insert

   GvpB
   

   Tags

   mCherry (K187N -please see depositor comments)

   Expression

   Mammalian

   Promoter

   TRE3G promoter

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV TRE-GFP-TMD-DLL1-Nbox-mutant

   Plasmid

   #194937

   Purpose

   LV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   eGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
   

   Use

   Lentiviral

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9-EF

   Plasmid

   #177267

   Purpose

   CRISPR vector

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY232

   Plasmid

   #184149

   Purpose

   short tracrRNA generator with WT anti-repeat region

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   s-tracrRNA-WT
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   araBAD promoter

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hygro-Bem1-mCherry-AID

   Plasmid

   #173925

   Purpose

   Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

   Depositor

   Liedwij Laan

   Article

   Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

   Inserts

   Partial Bem1 C-terminus
   hygromycin marker
   Bem1 downstream sequence
   

   Tags

   mCherry- AID

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

   Plasmid

   #73542

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

   Plasmid

   #73543

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2370-Tier1-PhCMV-TetR_F-type

   Plasmid

   #169573

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled transactivator
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEM262 - pDESTR4-R3 mosTI hygroR target

   Plasmid

   #159840

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pDESTR4-R3 mosTI hygroR target
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-9-35S-11

    Plasmid

    #176881

    Purpose

    MoClo Level 0 plasmid, contains 35S

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    35S terminator
    

    Expression

    Plant

    Mutation

    WT

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1

    Plasmid

    #182376

    Purpose

    Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    3xFlag
    mito-mTFP1
    

    Expression

    Mammalian

    Promoter

    CMV promoter and SV40 promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. NmMetNI-WT

    Plasmid

    #172114

    Purpose

    Expression of MetN and MetI membrane proteins

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Inserts

    MetN
    MetI
    

    Tags

    10x His Tag and Enterokinase

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAraQCas-BsaI

    Plasmid

    #170633

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt

    Plasmid

    #166993

    Purpose

    Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(0), Hairpin = 0 nt
    

    Expression

    Mammalian

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GLY, Linear-3, PolyU(4)

    Plasmid

    #166989

    Purpose

    Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(4)
    

    Expression

    Mammalian

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GLN, Linear-3, PolyU(4)

    Plasmid

    #166985

    Purpose

    Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(4)
    

    Expression

    Mammalian

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt

    Plasmid

    #166995

    Purpose

    Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(0), Hairpin = 0 nt
    

    Expression

    Mammalian

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. GP826

    Plasmid

    #164551

    Purpose

    Expresses Smo-FLAG K444, 448R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K444, 448R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    Two of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GP825

    Plasmid

    #164550

    Purpose

    Expresses Smo-FLAG K434, 448R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K434, 448R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    Two of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GP824

    Plasmid

    #164549

    Purpose

    Expresses Smo-FLAG K434, 444R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K434, 444R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    Two of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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