We narrowed to 10,611 results for: t7

1. pHB-2

   Plasmid

   #140955

   Purpose

   For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

   Depositor

   Kang Zhou

   Article

   Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

   Insert

   phaCAB
   

   Expression

   Bacterial

   Mutation

   R238S mutation in PhaC

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

   Plasmid

   #168192

   Purpose

   HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-LaG6-(EAAAK)4-OGT(4)
   

   Tags

   HA-Tag

   Expression

   Mammalian

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-BCA-Q11

   Plasmid

   #185781

   Purpose

   Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   BCA-Q11
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYCDuet_WTDHFR_WTTS

   Plasmid

   #91226

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLicC-MBP-APETx2

   Plasmid

   #72668

   Purpose

   Periplasmic expression of APETx2 in E.coli

   Depositor

   Glenn King

   Article

   Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

   Insert

   APETx2
   

   Tags

   His tag, MBP, MalE signal sequence for periplasmi…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE5400_pMBP_CDS dropout vector

   Plasmid

   #221189

   Purpose

   Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

   Depositor

   Tobias Erb

   Article

   Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

   Type

   Empty backbone

   Tags

   10x His-tag + E. coli maltose binding protein (MB…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xHis(TEV)-Drp1(K38A)-StrepII

   Plasmid

   #174423

   Purpose

   Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K38A

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDV5 containing Sb-convex

   Plasmid

   #132696

   Purpose

   pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_convex including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 GDI2

   Plasmid

   #67093

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GDI2 (GDI2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pMR-A-6

    Plasmid

    #138520

    Purpose

    P3d

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-M2-S2-S3-S4T3D

    Plasmid

    #33302

    Depositor

    Terence Dermody

    Article

    Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

    Inserts

    S2 (S2 Orthoreovirus T3D)
    S3 (S3 Orthoreovirus T3D)
    S4 (S4 Orthoreovirus T3D)
    M2 (M2 Orthoreovirus T3D)
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTU398

    Plasmid

    #178980

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    gRAMP
    

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. TrpLE-M-nMSP1E3D1

    Plasmid

    #179949

    Purpose

    Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

    Depositor

    Kerney Glover

    Article

    Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

    Insert

    TrpLE-M-nMSP1E3D1
    

    Tags

    TrpLE

    Expression

    Bacterial

    Mutation

    Native Trp residues have been mutated to Phe resi…

    Promoter

    T7

    Available Since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLP-pR-himar-apraR

    Plasmid

    #199996

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Expression

    Bacterial

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPTI-[5,55]Gly14Ile38

    Plasmid

    #195059

    Purpose

    Thermal stability of BPTI

    Depositor

    Yutaka Kuroda

    Article

    Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

    Insert

    bovine pancreatic trypsin inhibitor
    

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    Changed Alanine residues at position of 14 and 38…

    Promoter

    T7 promotor

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a His tag-FRB-Venus C

    Plasmid

    #100980

    Purpose

    Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    His-FRB-Venus C
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ABKAR(T/A)

    Plasmid

    #140052

    Purpose

    Negative control mutant for ABKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR(T/A)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Nov. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET26_MraYaa

    Plasmid

    #100166

    Purpose

    recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

    Depositor

    Seok-Yong Lee

    Article

    Chung et al Science. 2013 Aug 30;341(6149):1012-1016

    Insert

    mraY (phospho-MurNAc-pentapeptide translocase)
    

    Tags

    MBP-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPbuCas13b

    Plasmid

    #184565

    Purpose

    Inducible expression of PbuCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Cas13b nuclease from Prevotella buccae
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a(+) 6xHis-TEV-IFIT1

    Plasmid

    #53557

    Purpose

    bacterial expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only