Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,853 results for: REV

Showing: 11921 - 11960 of 18853 results

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pCA528 SPASTIN , F124D, residues 108-200

Plasmid

#180629

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, F124D

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , L177D, residues 108-200

Plasmid

#180634

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, L177D

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP8, residues 1-147

Plasmid

#180636

Purpose

Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP8 (USP8 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-147

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP54, residues 731-878

Plasmid

#180637

Purpose

Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP54 (USP54 Human)

Tags

HIS-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET16B VPS4A, residues 1-84

Plasmid

#180638

Purpose

Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4A (VPS4A Human)

Tags

His tag

Expression

Bacterial

Mutation

Residues 1-84

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, V18D

Plasmid

#180612

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, V18D mutation

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, F98D

Plasmid

#180613

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, F98D mutation

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMVQA

Plasmid

#197894

Purpose

pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

Depositor

Thomas Klimkait

Article

Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

Insert

HIV-1 3' end (HXB2: 9045 till poly-A(10)

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGA-red-mini

Plasmid

#196338

Purpose

Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGA-blue

Plasmid

#196336

Purpose

Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

Plasmid

#199447

Purpose

Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

dSaCas9-10xGCN4

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJR035

Plasmid

#198821

Purpose

For tissue specific RNA-seq

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::rpl-22HA-SL2-mKate::let-858 3'UTR

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shCse1l v1 puro

Plasmid

#192342

Purpose

Lentiviral expression vector for an inducible shCse1L v1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shCse1l v1 (Cse1l Budding Yeast)

Use

Lentiviral

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 1

Plasmid

#198501

Purpose

lentiviral stable expression of mNudcd3 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 2

Plasmid

#198502

Purpose

lentiviral stable expression of mNudcd3 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-2

Plasmid

#198476

Purpose

lentiviral stable expression of mRab12 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-1

Plasmid

#198475

Purpose

lentiviral stable expression of mRab12 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_578-675-GB1_AD

Plasmid

#148504

Purpose

Bacterial Expression of DmeIF4G_578-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-675 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_601-634-GB1_AD

Plasmid

#148505

Purpose

Bacterial Expression of DmeIF4G_601-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_601-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmeIF4G_601-675-GB1_AD

Plasmid

#148507

Purpose

Bacterial Expression of DmeIF4G_601-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_601-675 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmeIF4G_617-634-GB1_AD

Plasmid

#148508

Purpose

Bacterial Expression of DmeIF4G_617-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_617-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmeIF4G_617-675-GB1_AD

Plasmid

#148509

Purpose

Bacterial Expression of DmeIF4G_617-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_617-675 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-R249H_X

Plasmid

#147979

Purpose

Insect Expression of DmCaf40-R249H

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-R249H (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-Y208AR210AH213AA253F_X

Plasmid

#147980

Purpose

Insect Expression of DmCaf40-Y208AR210AH213AA253F

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-Y208AR210AH213AA253F (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-Y208AR249A_X

Plasmid

#147981

Purpose

Insect Expression of DmCaf40-Y208AR249A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-Y208AR249A (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCaf40-A253FY208A_X

Plasmid

#147992

Purpose

Insect Expression of DmCaf40-A253FY208A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-A253FY208A (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCaf40-R249H_X

Plasmid

#147994

Purpose

Insect Expression of DmCaf40-R249H

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-R249H (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCaf40-Y208AR249A_X

Plasmid

#147995

Purpose

Insect Expression of DmCaf40-Y208AR249A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-Y208AR249A (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-F65AC69Y_X

Plasmid

#147974

Purpose

Insect Expression of DmCaf40-F65AC69Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-F65AC69Y (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-N63AF65AC69Y_X

Plasmid

#147975

Purpose

Insect Expression of DmCaf40-N63AF65AC69Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-N63AF65AC69Y (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-N63AF65AC69YV76Y_X

Plasmid

#147976

Purpose

Insect Expression of DmCaf40-N63AF65AC69YV76Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-N63AF65AC69YV76Y (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-N63AF65AC69YV76YR210AH213A_X

Plasmid

#147977

Purpose

Insect Expression of DmCaf40-N63AF65AC69YV76YR210AH213A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-N63AF65AC69YV76YR210AH213A (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-NFCVYRHAtoAAYYAAAF_X

Plasmid

#147978

Purpose

Insect Expression of DmCaf40-NFCVYRHAtoAAYAAAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYRHAtoAAYAAAF (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCaf40-NFCVYAtoAAYYAF_W

Plasmid

#147829

Purpose

Insect Expression of DmCaf40-NFCVYAtoAAYYAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT-CTAP-PrP-SBP-DmCaf40_W

Plasmid

#147832

Purpose

Insect Expression of DmCaf40

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40 (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-NFCVYAtoAAYYAF_W

Plasmid

#147838

Purpose

Insect Expression of DmCaf40-NFCVYAtoAAYYAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCaf40-R210AH213A_W

Plasmid

#147839

Purpose

Insect Expression of DmCaf40-R210AH213A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-R210AH213A (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmeIF4G_578-634-GB1_AG

Plasmid

#148744

Purpose

Bacterial Expression of DmeIF4G_578-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Human, Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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