2,162 results

1. slc1a3b:myrGFP-P2A-H2AmCherry

   Plasmid

   #170208

   Purpose

   Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGFP-P2A-H2AmCherry

   Expression

   Mutation

   Promoter

   slc1a3b

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-nCas9n-nanos 3’UTR

   Plasmid

   #62542

   Purpose

   expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

   Insert

   nanos 3' UTR (nanos3 Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   3' UTR only

   Promoter

   SP6

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

   Plasmid

   #191500

   Purpose

   GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Creation of transgenic fish using tol2 transgenes…

   Tags

   Expression

   Mutation

   Promoter

   myo6b

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-elavl3

   Plasmid

   #82400

   Purpose

   Gateway-compatible zebrafish elavl3 promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   elavl3 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDESTtol2pACrymCherry

   Plasmid

   #64023

   Purpose

   Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

   Depositor

   Joachim Berger, Peter Currie

   Article

   Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

   Insert

   mCherry
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   alpha-Acrystallin promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol1:uas-myc-act-map2k2b-2a-tdTomato

   Plasmid

   #104721

   Purpose

   UAS-driven expression of activated MEK

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   activated map2k2b (map2k2b Zebrafish)
   

   Use

   Zebrafish transposon

   Tags

   2A-tdTomato and myc tag

   Expression

   Mutation

   serines 221 and 225 to aspartic acid

   Promoter

   10xUAS

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT-TODestGFP_RNAseH1

   Plasmid

   #65784

   Purpose

   expresses GFP-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Zebrafish, Human)
   

   Use

   Tags

   GFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-mnx1

   Plasmid

   #82402

   Purpose

   Gateway-compatible zebrafish mnx1 promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   mnx1 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME map2k2a_s219D

   Plasmid

   #201058

   Purpose

   Gateway middle entry vector expressing constitutively active zebrafish map2k2a

   Depositor

   Sarah Childs

   Article

   Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

   Insert

   MAP2k2a (map2k2a Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Mutation S219D, constitutively active

   Promoter

   none

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Plasmid

    #180475

    Purpose

    Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Zebrafish ubb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5E-kdrl

    Plasmid

    #78687

    Purpose

    kdrl promoter for Gateway cloning

    Depositor

    Sarah Childs, Jau-Nian Chen

    Article

    Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

    Insert

    kdrl promoter (kdrl Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    kdrl

    Available Since

    June 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-mpeg1.1

    Plasmid

    #75023

    Purpose

    Can be used to drive expression in zebrafish microglia.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    mpeg1.1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. punc503GFP

    Plasmid

    #64021

    Purpose

    503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expression

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    unc-45b: GFP (unc45b Zebrafish)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    503-bp promoter of zebrafish unc-45b

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2(+)-zGrad

    Plasmid

    #119716

    Purpose

    Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

    Depositor

    Holger Knaut

    Article

    Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

    Insert

    zGrad
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dat - EGFP - Tol2

    Plasmid

    #85418

    Purpose

    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

    Depositor

    Marc Ekker

    Article

    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

    Insert

    dopamine transporter (slc6a3 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    dat

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

    Plasmid

    #158962

    Purpose

    Cre-dependent Cas9-GFP expression

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    hsp70l promoter
    floxed DsRed cassette
    Cas9-GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74009

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5x UAS
    Cas9
    T2A
    GFP
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tol2-kdrl:mCherry-caax

    Plasmid

    #194287

    Purpose

    Expression plasmid for zebrafish or Danionella cerebrum

    Depositor

    Pui-Ying Lam

    Article

    Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

    Insert

    kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
    

    Use

    Zebrafish or danionella cerebrum expression

    Tags

    mCherryCAAX from the Tol2kit (Kwan et al., 2007)

    Expression

    Mutation

    Promoter

    kdrl(flk1)

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pfli1

    Plasmid

    #22413

    Depositor

    Nathan Lawson

    Article

    Lawson et al Development. 2001 Oct . 128(19):3675-83.

    Insert

    fli1 (fli1 Zebrafish)
    

    Use

    Riboprobe

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

20. 39 p ephrin B2

    Plasmid

    #13063

    Depositor

    Nathan Lawson, Michael Tsang

    Article

    Lawson et al Development. 2001 Oct . 128(19):3675-83.

    Insert

    ephrin B2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2010

    Availability

    Academic Institutions and Nonprofits only