2,159 results
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Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)PromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCS2-nCas9n-nanos 3’UTR
Plasmid#62542Purposeexpression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafishDepositorInsertnanos 3' UTR (nanos3 Zebrafish)
UseCRISPRTagsExpressionMutation3' UTR onlyPromoterSP6Available sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-cytoBirA-2A-mCherry_Ras
Plasmid#80062PurposeSox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGatewayTagsExpressionMutationPromoteralpha-Acrystallin promoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependent Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP-IRES2-EGFP
Plasmid#80333PurposeExpresses Danio rerio VSP with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP(C302S)-IRES2-EGFP
Plasmid#80331PurposeExpresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationC302SPromoterCMVAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hsp70l: hAPOB48-mCherry.
Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryExpressionMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME Integrin beta 1b
Plasmid#105976Purposeentry clone for gateway cloning, Full length Integrin beta 1b without stopDepositorInsertIntegrinbeta1b
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi
Plasmid#27320DepositorInsertzebrafish ubiquitin promoter (ubb Zebrafish)
UseMultisite gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bP1Egfp
Plasmid#90158Purposecontains cldn5b specific gene promoter driving expression of eGFPDepositorInsertcldn5bP1Egfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDESTtol2pACrymCherry
Plasmid#64023PurposeGateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lensDepositorInsertmCherry
UseGatewayTagsExpressionMutationPromoteralpha-Acrystallin promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Acvr1ba-P2A-Acvr2ba
Plasmid#232640PurposeOptogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expressionDepositorInsertAcvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
UseTagsExpressionMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCK036 desmaMCS:minCerulean,cryaa:Venus
Plasmid#195981PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pME-Trpa1b-2A-mCherry
Plasmid#106291PurposeGateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherryDepositorInsertTrpa1b (trpa1b Zebrafish)
UseGateway middle entryTags2A-mCherryExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-E1b:eGFP-Ds
Plasmid#102417PurposeFluorescent vector for screening enhancer elements in zebrafishDepositorInsertE1b:eGFP
UseTagsExpressionBacterialMutationPromoterZebrafish E1b minimal promoterAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only