1,878 results

1. pHW394 (15xUAS::GFP::let-858 3'UTR)

   Plasmid

   #85584

   Purpose

   cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

   Depositor

   Paul Sternberg

   Article

   Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   15xUAS-delta pes-10-GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   GFP(S65C) with three introns for expression in C.…

   Promoter

   15xUAS-delta pes-10-GFP

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS485

   Plasmid

   #188890

   Purpose

   Worm Cas9 expression vector, Peft-3 promoter

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Peft-3::Cas9
   

   Use

   Tags

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Prab-3::NLS::GFP

   Plasmid

   #68120

   Purpose

   Nuclear Localized GFP for pan-neuronal expression in C. Elegans

   Depositor

   Andrew Leifer

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

   Insert

   GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDO068

   Plasmid

   #198788

   Purpose

   Drives specific expression in the BAG neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Pgcy-33-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ108 - pENTR221 - [cbr-unc-119(+)]

   Plasmid

   #200367

   Purpose

   cbr-unc-119 rescue gene for use as co-injection marker.

   Depositor

   Christian Froekjaer-Jensen

   Article

   AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

   Insert

   [cbr-unc-119(+)]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ782 - HygroR

   Plasmid

   #190933

   Purpose

   Hygromycin resistance co-injection marker

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Hygromycin resistance gene
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-0

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTN4

   Plasmid

   #174212

   Purpose

   Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Inserts

   AtTIR1(F79G)::mRuby (TIR1 Nematode)
   GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   mRuby

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SL2 GFP H2B

   Plasmid

   #227717

   Purpose

   Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   SL2 GFP H2B (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSM.wrmScarlet1-10::SL2::GFP1-10

   Plasmid

   #234318

   Purpose

   For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

   Insert

   wrmScarlet1-10::SL2::GFP1-10
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

    Plasmid

    #129774

    Purpose

    Marker of microtubule dynamics in C. elegans hypodermis

    Depositor

    Andrew Chisholm

    Article

    Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

    Inserts

    Pcol-19
    ebp-2 (ebp-2 Nematode)
    unc-54 3'UTR
    

    Use

    Synthetic Biology; Gateway

    Tags

    Synthetic Intron and mGFP

    Expression

    Worm

    Mutation

    Promoter

    Pcol-19

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH4.66

    Plasmid

    #26951

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    beta-tubulin (tbb-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TC374

    Plasmid

    #164886

    Purpose

    Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rpl28-rtTA(Q)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEM318 - sgRNA mosTI unc-119 single-copy

    Plasmid

    #159822

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 single-copy
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TC358

    Plasmid

    #164885

    Purpose

    TRE::GFP, inducible GFP expression when co-express with rtTA

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ151 - ttTi5605_MCS

    Plasmid

    #19330

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    ttTi5605 targeting region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJG103

    Plasmid

    #158533

    Purpose

    wrmScarlet11 x3 tandem repeats

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet11 x3 tandem repeats
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH2930

    Plasmid

    #175283

    Purpose

    Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-16a (daf-16 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pges-1

    Available Since

    Dec. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pJH2198

    Plasmid

    #179773

    Purpose

    Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

    Depositor

    Mei Zhen

    Article

    Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

    Insert

    NLF-1::GFP (nlf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Prgef-1

    Available Since

    March 14, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLT 651

    Plasmid

    #59998

    Purpose

    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

    Depositor

    Lisa Timmons

    Article

    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

    Inserts

    klp-16 (klp-16 Nematode)
    him-8 (him-8 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    partial genomic

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOD2044-intDEG

    Plasmid

    #89366

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Expression

    Mutation

    Promoter

    Pelt-2

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. Punc-4::NGL-1::spGFP1-10

    Plasmid

    #65827

    Purpose

    For trans-synaptic labeling

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    NLG-1 (nlg-1 Nematode)
    

    Use

    Tags

    spGFP1-10

    Expression

    Worm

    Mutation

    Promoter

    Punc-4

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGLOW79

    Plasmid

    #177339

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pOD2045-intControl

    Plasmid

    #100005

    Purpose

    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Inserts

    zif-1 (zif-1 Nematode)
    unc-119
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJL43.1 - Pglh-2::MosTase::glh-2utr

    Plasmid

    #19332

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pglh-2::Mos1 transposase
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET_SZ1-RGG-RGG

    Plasmid

    #124942

    Purpose

    Expression of SZ1-RGG-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

    Plasmid

    #159798

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMA122 - peel-1 negative selection

    Plasmid

    #34873

    Purpose

    Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    peel-1 (peel-1 Nematode)
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. L4440_BioBrick-hif-1-sgRNA-A

    Plasmid

    #177784

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hif-1 (hif-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMDJ231 - Phsp Cas9

    Plasmid

    #191382

    Purpose

    Heat shock inducible Cas9 expression in C. elegans.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Phsp-16.41 Cas9 gpd-2 tagRFP-t
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Phsp-16.41

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge