We narrowed to 4,347 results for: ara-2

1. pCRISPomyces-AsCas12j-2

   Plasmid

   #191655

   Purpose

   All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

   Depositor

   Fong Tian Wong

   Article

   Tan et al bioRxiv 2021.10.28.465406

   Insert

   AsCas12j-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2 gRNA concatmer

   Plasmid

   #84879

   Purpose

   Empty concatmer vector in which 2 gRNAs can be inserted

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pCEP4 Bcl-2

   Plasmid

   #16461

   Depositor

   Bert Vogelstein

   Article

   Pietenpol et al Cancer Res. 1994 Jul 15. 54(14):3714-7.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pBzCas13b-gRNA-2

   Plasmid

   #164859

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLsCas13a-gRNA-2

   Plasmid

   #164866

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_003_sgTFAP4 guide 2

   Plasmid

   #193587

   Purpose

   TFAP4 knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgTFAP4 guide 2 (TFAP4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hNedd4-2 CS

   Plasmid

   #86041

   Purpose

   full length NeddL3 with C to S mutation with His Xpress tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

   Insert

   hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   C942S mutation in HECT domain

   Promoter

   CMV

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY71-tet.I.2

   Plasmid

   #133524

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_BRD003 sgKEAP1-2

   Plasmid

   #74986

   Purpose

   sgKEAP1-2, puromycin selection

   Depositor

   William Hahn

   Article

   Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

   Insert

   KEAP1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. EPHB4-gRNA1/2

    Plasmid

    #139974

    Purpose

    gRNA used for knockin EGFP into EPHB4 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    EPHB4 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. RhoB gRNA #2

    Plasmid

    #198722

    Purpose

    Cas9-mediated knockout of RhoB in mammalian cells #2

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoB (Rhob Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. RhoC gRNA #2

    Plasmid

    #198725

    Purpose

    Cas9-mediated knockout of RhoC in mammalian cells #2

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoC (Rhoc Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSPL3-ABCC8-2

    Plasmid

    #135916

    Purpose

    Encodes ABCC8 exon 2 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIII CKBs CRISPR with guides at 1-2 and 2-3

    Plasmid

    #238525

    Purpose

    Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha3

    Depositor

    Michael Hothorn

    Insert

    AtCK2alpha3 (AT2G23080 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmOrange 2-Actin

    Plasmid

    #199703

    Purpose

    visualization of cleavage furrow in living mammalian mitotic cells

    Depositor

    Kenji Sugimoto

    Article

    Tanaka et al Bioimages 25: 7-19, 2017

    Insert

    human cytoplasmic action
    

    Use

    Tags

    mOrange 2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDTnLAPP2A frame 2

    Plasmid

    #28228

    Depositor

    Harald von Melchner

    Article

    Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

    Insert

    Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
    

    Use

    Retroviral

    Tags

    EGFP and nLAP-tag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L-NARS-2

    Plasmid

    #166532

    Purpose

    scFv of a human scaffold targeting Asparaginyl-tRNA synthetase. Antigen coverage aa 1-458 of 458, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-(-2)AP-Ub

    Plasmid

    #232582

    Purpose

    Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330A-Prdm14-L5-#2

    Plasmid

    #171514

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330A-Prdm14-L3-#2

    Plasmid

    #171510

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX330A-Prdm14-L4-#2

    Plasmid

    #171512

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRPa-iSL(MCS1+2)

    Plasmid

    #69244

    Purpose

    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    Expression

    Mutation

    Promoter

    RRNA promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330A-Blimp1-L1-#2

    Plasmid

    #171504

    Purpose

    deletion of a genomic locus in Blimp1(Prdm1) gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Blimp1 (Prdm1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX330A-Blimp1-L2-#2

    Plasmid

    #171506

    Purpose

    deletion of a genomic locus in Blimp1(Prdm1) gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Blimp1 (Prdm1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX330A-Prdm14-ex1-#2

    Plasmid

    #171508

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMDC32B-AtMIR390a-NbSu-2

    Plasmid

    #213400

    Purpose

    Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

    Insert

    Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTwist-SARS-CoV-2 Δ18 P.2/B.1.1.248

    Plasmid

    #173477

    Purpose

    Encodes SARS-CoV-2 P.2/B.1.1.248 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 Spike Truncated to remove 18 amino acids
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mTALEAD ATP6-2-1

    Plasmid

    #210829

    Purpose

    mTALEAD expression vector

    Depositor

    Shin-ichi Arimura

    Article

    Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

    Insert

    For specific A-to-G base editing to introduce targeted A-to-G mutation in ATP6-2 in mitochondria
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mTALEAD 16S rRNA-2

    Plasmid

    #210834

    Purpose

    mTALEAD expression vector

    Depositor

    Shin-ichi Arimura

    Article

    Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

    Insert

    For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVY-2 sfGFP-tag2

    Plasmid

    #194617

    Purpose

    fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

    Insert

    sfGFP-K(GGSKKRKKR)2
    

    Use

    Tags

    KGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX CnA 2-347

    Plasmid

    #13251

    Depositor

    Anjana Rao

    Article

    Li et al J Mol Biol. 2004 Oct 1. 342(5):1659-74.

    Insert

    CnA (PPP3CA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 2-347 (truncated before B binding hel…

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

32. pro::EX1-2:GFP

    Plasmid

    #218565

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAGM9121_Sce-Invertase 2 SP

    Plasmid

    #153493

    Purpose

    S. cerevisiae Invertase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Invertase 2 SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLL3.7 Alk.2 shRNA

    Plasmid

    #59297

    Purpose

    shRNA to mouse Alk

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

    Insert

    Alk.2 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

35. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid

    #70657

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

36. Lenti-sgSetd2#2/Cre

    Plasmid

    #89650

    Purpose

    Expresses a Setd2-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Setd2 (Setd2 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lentiCRISPR - C9orf114 sgRNA 2

    Plasmid

    #70654

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against C9orf114
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pXPR_003 sgPIGA guide 2

    Plasmid

    #193611

    Purpose

    PIGA knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgPIGA guide 2 (PIGA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXPR_003 sgPIGW guide 2

    Plasmid

    #193613

    Purpose

    PIGW knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgPIGW guide 2 (PIGW Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pXPR_003 sgNFkBIA guide 2

    Plasmid

    #193596

    Purpose

    NFkBIA knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgNFkBIA guide 2 (NFKBIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge