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Showing: 101 - 150 of 253 results

  3. PL-5LTR-GW-A

    Plasmid
    #138480
    Purpose
    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.
    Depositor
    Akitsu Hotta
    Article
    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:
    Insert
    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138480-plasmid-map-sequence-id-275574

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  11. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Use
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

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  12. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Use
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

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  13. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Article
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Promoter
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

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  20. pNS19-LV-mutRFP-2A-GFP

    Plasmid
    #113716
    Purpose
    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)
    Depositor
    Gerald Schwank
    Article
    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1
    Insert
    mutRFP-2A-GFP-IRES-Puro
    Use
    Lentiviral; Reporter system
    Tags
    Expression
    Mammalian
    Mutation
    Y64L (mutRFP)
    Promoter
    Available since
    Aug. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113716-plasmid-map-sequence-id-220030

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  40. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 and tcp830
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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  50. pLEX307_CR-PDHA2S291D

    Plasmid
    #115200
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    115200-plasmid-map-sequence-id-232426

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Showing: 101 - 150 of 253 results