We narrowed to 126 results for: pj sac

1. pCMV-SaPE2-P2A-BSD

   Plasmid

   #174818

   Purpose

   Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   SaPE2-P2A-BSD
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSG-3'Hu-RelA

   Plasmid

   #27276

   Depositor

   Thomas Gilmore

   Article

   Morin et al Nucleic Acids Res. 1993 May 11. 21(9):21

   Insert

   RelA (RELA Human)
   

   Use

   Tags

   GAL4 (aa1-147)

   Expression

   Mammalian

   Mutation

   Contains only the transactivation domain (aa 292-…

   Promoter

   Available since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pHPHS232

   Plasmid

   #228356

   Purpose

   Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

   Inserts

   mTagBFP2
   Reverse tetracycline transactivator 3
   neo
   

   Use

   Tags

   Blasticidin S deaminase and iCasp9

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV and gene trap

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDH-EFIa-Id1-PGK-puro

   Plasmid

   #107735

   Purpose

   Expresses Id1 in mammalian cells

   Depositor

   Alexandre Colas

   Article

   Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

   Insert

   Id1 (T Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTT021

   Plasmid

   #226782

   Purpose

   Expression of wzxE and other genes from the E. coli enterobacterial common antigen biosynthesis gene cluster

   Depositor

   Chris Whitfield, James Naismith

   Article

   Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

   Insert

   wzxE, wecF, wzyE, wecG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BAD

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-EF1a-DIO-FLPo-Myc

   Plasmid

   #124641

   Purpose

   AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   FLPo
   

   Use

   AAV and Cre/Lox

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

   Plasmid

   #205540

   Purpose

   Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Gag-MCP
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   TRE3GS

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD-WzxE-TEV-His10

   Plasmid

   #226781

   Purpose

   Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

   Depositor

   James Naismith

   Article

   Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

   Insert

   wzxE
   

   Use

   Tags

   TEV cleavable 10xHistidine tag

   Expression

   Bacterial

   Mutation

   valine insertion in position 2 due to the cloning…

   Promoter

   araBAD

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

   Plasmid

   #112021

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta/alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

    Plasmid

    #112022

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO alpha/beta into TRBC1 HDRDT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NYESO alpha into TRAC HDRT Source (pTR 223)

    Plasmid

    #112023

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NYESO beta into TRBC1 HDRT Source (pTR 277)

    Plasmid

    #112024

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta into TRBC1 HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RAB11A-GFP HDRT Source (pTR 143)

    Plasmid

    #112012

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-GFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CD4-C-GFP HDRT Source (pTR 152)

    Plasmid

    #112018

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-GFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CLTA-GFP HDRT Source (pTR 153)

    Plasmid

    #112016

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-GFP HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RAB11A-mCherry HDRT Source (pTR 144)

    Plasmid

    #112013

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-mCherry HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CLTA-mCherry HDRT Source (pTR 177)

    Plasmid

    #112017

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-mCherry HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BATF-GFP HDRT Source (pTR 146)

    Plasmid

    #112015

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    BATF-GFP HDRT (BATF Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RAB11A-BFP HDRT Source (pTR 145)

    Plasmid

    #112014

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-BFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CD4-C-BFP HDRT Source (pTR 176)

    Plasmid

    #112020

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-BFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CD4-C-mCherry HDRT Source (pTR 175)

    Plasmid

    #112019

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-mCherry HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC19_18GZG

    Plasmid

    #117228

    Purpose

    shble flanked by an endogenous GAPDH promoter and terminator system

    Depositor

    Jackie Collier

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    shble
    

    Use

    Thraustochytrid expression

    Tags

    Expression

    Mutation

    Promoter

    GAPDH

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. DinoIII-neo

    Plasmid

    #155026

    Purpose

    Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

    Depositor

    Senjie Lin

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    amino 3'-glycosyl phosphotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. DinoIII-pat

    Plasmid

    #155028

    Purpose

    Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

    Depositor

    Senjie Lin

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    Phosphinothricin N-acetyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. DinoIII-bsr

    Plasmid

    #155027

    Purpose

    Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

    Depositor

    Senjie Lin

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    Blasticidin-S deaminase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC:FCP:ShBle:FCP:EGFP

    Plasmid

    #85987

    Purpose

    Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

    Depositor

    Thomas Mock

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Inserts

    ShBle
    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    FCP

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge