We narrowed to 29,673 results for: PLE

1. pLentiDP1.0

   Plasmid

   #171094

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX_306

   Plasmid

   #41391

   Purpose

   Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

   Depositor

   David Root

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiGuide

   Plasmid

   #117986

   Purpose

   pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

   Depositor

   Paul Khavari

   Article

   Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

   Insert

   sgRNA backbone (F+E)
   

   Use

   Lentiviral

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLENTI_hACE2_HygR

   Plasmid

   #155296

   Purpose

   Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

   Depositor

   Raffaele De Francesco

   Article

   Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

   Insert

   hACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   C9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLEX305_3xFLAG_RELA1

   Plasmid

   #220324

   Purpose

   Expresses full length human RELA with an N-terminal 3xFLAG tag

   Depositor

   Jonathan Brown

   Insert

   RELA (RELA Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Promoter

   hPGK

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiRNAGuide_003

   Plasmid

   #192505

   Purpose

   For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

   Insert

   hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti NL

   Plasmid

   #113450

   Purpose

   Lentiviral NanoLuc control expression vector

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   NanoLuc
   

   Use

   Lentiviral and Luciferase

   Tags

   cmyc

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPR_Puro_sgTP53

   Plasmid

   #187819

   Purpose

   pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgTP53
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLENTI_RPS10

   Plasmid

   #127129

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   RPS10 (RPS10 Human)
   

   Use

   Lentiviral

   Mutation

   K24R- please see depositor comments

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.2_mTurquoise2

    Plasmid

    #113723

    Purpose

    Expresses the fluorescent protein mTurquoise2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PlepA

    Plasmid

    #55175

    Purpose

    Strong constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    lepA promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti_EF1a_Flag_TP53_R248W

    Plasmid

    #183114

    Purpose

    Expresses flag-tagged p53 with R248W mutation in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 248 to tryptophan

    Promoter

    EF1a

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_C_Myc_DDK_IRES_Puro_PRKRA

    Plasmid

    #106113

    Purpose

    Lentiviral expression of PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and Myc

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. h-Plekhm1_EGFP

    Plasmid

    #73836

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiGuide_GFP_NT135

    Plasmid

    #183118

    Purpose

    pLentiGuide modified to express GFP and a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR_mCherry

    Plasmid

    #229078

    Purpose

    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    EF1s

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCMVblast_SLC38A2_wt

    Plasmid

    #156180

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA se…

    Promoter

    CMV

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPR_zeo_sgNT135

    Plasmid

    #183181

    Purpose

    pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

    Plasmid

    #106120

    Purpose

    Lentiviral expression of ALDH7A1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    ALDH7A1 (ALDH7A1 Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and Myc

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

    Plasmid

    #211471

    Purpose

    lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    Cas9_T2A_mNeonGreen_P2A_BlasticidinR
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    hU6, EF-1a

    Available Since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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