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pLL5.0_shRNAHsCavin1_DrCavin1b_EGFP
Plasmid#229690PurposeLentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish Cavin1 (Cavin1 rescue)DepositorUseLentiviralTagsEGFPExpressionMutationPromoterLTR viral promoterAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp_CamRcargo
Plasmid#212143PurposePreparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
NarCas9_AAV
Plasmid#192145PurposeExpresses NarCas9?and cloning backbone for sgRNADepositorInsertNarCas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCAS.Sfi
Plasmid#196377PurposeEmpty avian expression vector RCAS with modifications for fixed orientation cloning into SfiI sitesDepositorTypeEmpty backboneUseRetroviral; AvianTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
InterCatch
Plasmid#186906PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BRCA1
Plasmid#115491PurposeEGFP-BRCA1 expression vector. Parton clone BXYDepositorInsertBRCA1 (BRCA1 Human)
UseTagsEGFPExpressionMammalianMutationS1009R (See depositor comments)PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-SMARCA4
Plasmid#65391PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
WT Smarca4-sfGFP
Plasmid#107056PurposeExpression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationwild-typePromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIN BRCA2 WT
Plasmid#16245DepositorInsertBRCA2 (BRCA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR
Plasmid#64927PurposeFRET-based biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR (EIF4EBP1 Synthetic, Human)
UseTagsCerulean and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRCaMP1b.WPRE.SV40
Plasmid#100851PurposeAAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertjRCaMP1b
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR(T/A)
Plasmid#64928PurposeNegative-control mutant for TORCAR biosensor.DepositorInsertTORCAR(T/A) (EIF4EBP1 Synthetic, Human)
UseTagsCerulean and YPetExpressionMammalianMutation4EBP1 domain contains T37A and T46A mutations.PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SMARCA2-GFP
Plasmid#65390PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSMARCA2 (SMARCA2 Human)
UseTagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCAS GFP (CT#22)
Plasmid#13878DepositorInsertGFP
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro HA Brca1
Plasmid#14999DepositorInsertBrca1 (BRCA1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shBRCA1 #1
Plasmid#44594DepositorInsertBRCA1 (Brca1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shBRCA1 #2
Plasmid#44595DepositorInsertBRCA1 (Brca1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT3TS nErCas12an
Plasmid#132642PurposeUsed for in vitro transcription of dual NLS ErCas12a mRNADepositorInsertErCas12a
UseCRISPR; In vitro transcriptionTagsSV40 NLSExpressionMutationPromoterT3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
