We narrowed to 2,461 results for: T2A

1. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

   Plasmid

   #204151

   Purpose

   Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   SARS-CoV-2 Delta variant spike
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   21 amino acid cytoplasmic tail deletion

   Promoter

   TRE3G

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro

   Plasmid

   #168807

   Purpose

   PiggyBac transposon construct with doxycycline inducible MYOD1, BAF60C + mCherry expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   MYOD1 (MYOD1 Synthetic, Human)
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_TagBFP_P2AT2A_GFP-nb-KS

   Plasmid

   #238274

   Purpose

   For overexpression of TagBFP_P2AT2A_GFP-nb-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TagBFP_P2AT2A_GFP-nb-KS
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5_TagBFP_P2AT2A_GFP-nb

   Plasmid

   #238273

   Purpose

   For overexpression of TagBFP_P2AT2A_GFP-nb

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TagBFP_P2AT2A_GFP-nb
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

   Plasmid

   #211471

   Purpose

   lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   Cas9_T2A_mNeonGreen_P2A_BlasticidinR
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   hU6, EF-1a

   Available Since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45565

   Purpose

   Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   I-Sce I Nuclease
   BFP
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. CDK1as_T2A_puromycin

   Plasmid

   #118595

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45564

   Purpose

   Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   d14 eGFP donor
   I-Sce I Nuclease
   BFP
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted n' 15 amino acids

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDK1as_T2A_Zeo

   Plasmid

   #118596

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2ADWpuro_2paRAF

    Plasmid

    #129653

    Purpose

    For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCYT2A

    Plasmid

    #42415

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PCYT2A (PCYT2 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. XClone_Cas9_KRAB_T2A_BFP

    Plasmid

    #141208

    Purpose

    Tunable and temporal expression control of dCas9

    Depositor

    Rory Johnson

    Insert

    dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid

    #200910

    Purpose

    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

    Depositor

    Debojyoti Chakraborty

    Insert

    3XHA_den1FnCas9_KRAB_eGFP
    

    Use

    CRISPR

    Tags

    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Glutamic Acid to Arginine at 1369 position

    Promoter

    CBH

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MT2a

    Plasmid

    #105693

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 2a

    Depositor

    Artur Krężel

    Article

    Drozd et al Metallomics. 2018 Apr 25;10(4):595-613.

    Insert

    Metallothionein 2a (MT2A Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. KAT2A

    Plasmid

    #38914

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KAT2A (KAT2A Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pT2ADW_2paRAF

    Plasmid

    #129654

    Purpose

    For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWPI_GW_BioID2_T2A_Blast

    Plasmid

    #214831

    Purpose

    Destination vector for cloning BioID2 fusion proteins

    Depositor

    Hani Goodarzi

    Article

    Khoroshkin et al Nat Commun. 2024 Sep 9;15(1):7872. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    BioID2

    Expression

    Bacterial and Mammalian

    Available Since

    Aug. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGD_MUTT2A_E

    Plasmid

    #218899

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    MUTT2A
    

    Use

    Synthetic Biology

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CL20_mEGFP_KMT2A_EPS15

    Plasmid

    #205841

    Purpose

    Express mEGFP-tagged fusion protein, KMT2A_EPS15 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    KMT2A_EPS15 (KMT2A, EPS15 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid

    #200389

    Purpose

    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

    Depositor

    Debojyoti Chakraborty

    Insert

    PX458_3XHA_dFnCas9_KRAB_eGFP
    

    Use

    CRISPR

    Tags

    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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