We narrowed to 6,172 results for: nls

1. HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

   Plasmid

   #222994

   Purpose

   Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CMV-NLS-dCas9-NLS

   Plasmid

   #99908

   Purpose

   For the expression of NLS-dCas9-NLS

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-dCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-NLSmCherryNLS Clover-HDHB

   Plasmid

   #190223

   Purpose

   Fluorescent cell cycle reporter

   Depositor

   Laura Heiser

   Article

   Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

   Inserts

   Human DNA Helicase B (amino acids 994-1087) (HELB Human)
   NLS-mCherry-NLS-P2A-Puromycin
   

   Use

   Transposon

   Tags

   Clover fluorescent protein

   Expression

   Mammalian

   Mutation

   Amino Acids 994-1087

   Promoter

   EF1a and RPBSA

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

   Plasmid

   #222993

   Purpose

   Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

   Plasmid

   #222991

   Purpose

   Bacterial expression of iGeoCas9(C2) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

   Plasmid

   #222992

   Purpose

   Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-1BPNLS-Cas9-1BPNLS

   Plasmid

   #87108

   Purpose

   BPNLS-Cas9-BPNLS expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV_NLS-SaCas9-NLS-VPR

   Plasmid

   #68496

   Purpose

   AAV vector containing SaCas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   SaCas9-VPR
   

   Use

   AAV and CRISPR

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUB-nHyPer2 (NLS:HyPer2:NLS)

   Plasmid

   #84730

   Purpose

   Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   HyPer2 targeted to nucleus
   

   Use

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Mutation

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid

    #107305

    Purpose

    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2x NLS and FKBP

    Expression

    Mammalian

    Mutation

    R1335K and fused to FKBP

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS

    Plasmid

    #244837

    Purpose

    Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR

    Tags

    2xSV40 NLS

    Expression

    Mammalian

    Mutation

    G146R/R182V/E795Q

    Promoter

    T7

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

    Plasmid

    #244844

    Purpose

    Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    iCas12a
    

    Use

    CRISPR

    Tags

    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

    Expression

    Bacterial

    Mutation

    F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…

    Promoter

    T7

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM5_CL7_Bac_2NLS-Lbm6 (LbCas12a)-4NLS

    Plasmid

    #244840

    Purpose

    Bacterial expression of Lbm6 LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    Lbm6-Cas12a
    

    Use

    CRISPR

    Tags

    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

    Expression

    Bacterial

    Mutation

    D535G/S551F/D665N

    Promoter

    T7

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEM6_CL7_Bac_2NLS-RVQ (LbCas12a)-2NLS

    Plasmid

    #244841

    Purpose

    Bacterial expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR

    Tags

    10xHis-CL7-2xSV40 NLS and 2xSV40 NLS

    Expression

    Bacterial

    Mutation

    G146R/R182V/E795Q

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

    Plasmid

    #222990

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
    

    Use

    CRISPR

    Tags

    CL7, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

    Plasmid

    #176270

    Purpose

    Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    TadA-TadA*(8e)-dCasMINI-V4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. nls-transposase-nls-pA in pT3TS-Dest

    Plasmid

    #140873

    Purpose

    Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nls-transposase-nls (Tol2)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

    Plasmid

    #132405

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-NLS-dCas13-NLS-eNAT10

    Plasmid

    #238300

    Purpose

    Expresses nuclear version of the programmable RNA acetylation system.

    Depositor

    Won Do Heo

    Article

    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

    Insert

    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    

    Use

    CRISPR

    Tags

    SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

    Expression

    Mammalian

    Mutation

    H133A for catalytically inactive mutant, deletion…

    Promoter

    Available Since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

    Plasmid

    #244838

    Purpose

    Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    Flex-Cas12a
    

    Use

    CRISPR

    Tags

    2xSV40 NLS

    Expression

    Mammalian

    Mutation

    G146R/R182V/D535G/S551F/D665N/E795Q

    Promoter

    T7

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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