We narrowed to 223 results for: stem loop

1. pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104096

   Purpose

   Expresses TREAT-ARE reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Tags

   12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

   Expression

   Mammalian

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mAIRN HO

   Plasmid

   #207411

   Purpose

   Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   mAIRN
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mAIRN CD

   Plasmid

   #207412

   Purpose

   Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   mAIRN
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ECFP HO

   Plasmid

   #207413

   Purpose

   Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   ECFP
   

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ECFP CD

   Plasmid

   #207414

   Purpose

   Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   ECFP
   

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LsgRNA-MS2

   Plasmid

   #235597

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-Osp.gRNA-MS2in

   Plasmid

   #229772

   Purpose

   Contains the U6 promoter and an optimized gRNA backbone inserted with two copies of the MS2 stem loop.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

   Insert

   gRNA cassette with two MS2 stem loops
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104140

   Purpose

   Expresses TREAT-5'TOP reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Tags

   24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

   Expression

   Mammalian

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SARS-CoV-2 5'UTR-Luciferase

    Plasmid

    #191480

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR
    

    Use

    Lentiviral and Luciferase

    Mutation

    contains SARS-CoV-2 5' UTR

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-mScarlet-SARS-CoV-2-SL1 alone

    Plasmid

    #191485

    Purpose

    Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

    Plasmid

    #191484

    Purpose

    Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Dest-MBP-Nsp1

    Plasmid

    #191483

    Purpose

    Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG86-J23151-LasR

    Plasmid

    #102456

    Purpose

    Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    LasR (lasR )
    

    Expression

    Bacterial

    Promoter

    J23151

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSG22-pBAD-TetR

    Plasmid

    #102451

    Purpose

    Expresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    TetR (tetR )
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSG28-J23151-AraC

    Plasmid

    #102453

    Purpose

    Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    AraC (araC )
    

    Expression

    Bacterial

    Promoter

    J23151

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSG15-pBAD-tetO-deGFP

    Plasmid

    #102450

    Purpose

    Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    deGFP
    

    Expression

    Bacterial

    Promoter

    pBAD-tetO

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MK1283

    Plasmid

    #71428

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Mutation

    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MK1274

    Plasmid

    #71431

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Mutation

    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSG84-pLas-TetRssrA

    Plasmid

    #102455

    Purpose

    Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    TetR (tetR )
    

    Tags

    ssrA

    Expression

    Bacterial

    Promoter

    pLas

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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