We narrowed to 3,272 results for: aaas

1. pMH-25

   Plasmid

   #158436

   Purpose

   pBAD33 vector encoding MotA, YPet - 3 x EAAAK linker - MotB

   Depositor

   Francesco Pedaci

   Article

   Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

   Inserts

   MotA
   MotB
   

   Use

   Tags

   yPET - 3 x EAAAK linker

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMH-05

   Plasmid

   #158435

   Purpose

   pBAD33 vector encoding MotA, YPet - EAAAK linker - MotB

   Depositor

   Francesco Pedaci

   Article

   Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

   Inserts

   MotA
   MotB
   

   Use

   Tags

   yPET - EAAAK linker

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PX458_ZBTB1_1

   Plasmid

   #128242

   Purpose

   Encodes gRNA for 3' target of human ZBTB1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB1 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCR3.1-OPNc M1

   Plasmid

   #106321

   Purpose

   Mammalian expression of mutant osteopontin

   Depositor

   Georg Weber

   Article

   Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

   Insert

   OPNc (SPP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   OPNc mutated splice site EEKQ-->AAAA

   Promoter

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330_DDX4-cterm

   Plasmid

   #222520

   Purpose

   Cas9/sgRNA plasmid for targeting DDX4

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   Cas9, DDX4 sgRNA (DDX4 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCR-Hyg

   Plasmid

   #158708

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Hsp60

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR-Zeo

   Plasmid

   #158709

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Hsp60

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. OgeuIscB ωRNA-v13

   Plasmid

   #219634

   Purpose

   human U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloning

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

   Insert

   OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC101-GFPmut2-mScarlet-I A4

   Plasmid

   #208193

   Purpose

   frameshift mistranslation, A4

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAA

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hRIP3 GFP RHIM mut

    Plasmid

    #41385

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (RIPK3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RHIM mutant (AAAA-459-462)

    Promoter

    CMV

    Available since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

11. PX458 pSp Cas9 Rhino1 tagA

    Plasmid

    #211627

    Purpose

    Cas9 Rhino1 tagA

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    RHNO tagA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pColE1_sgRNA_T2_AmpR

    Plasmid

    #119159

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pColE1_sgRNA_T3_AmpR

    Plasmid

    #119160

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pColE1_sgRNA_T1_AmpR

    Plasmid

    #119158

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-EPN-01(ΔL1)

    Plasmid

    #80049

    Purpose

    Expression of EPN-01 mutated in TSG101 binding site.

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01(deltaPTAP)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PTAP10AAAA

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-U6-AasgRNA3.8

    Plasmid

    #121958

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, short version
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pALPS puro miR30-STAT1

    Plasmid

    #117160

    Purpose

    Target site ATATCCAGTTCCTTTAGGGCC

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTGMP-p16/p19.478

    Plasmid

    #32717

    Depositor

    Scott Lowe

    Article

    Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

    Insert

    p16/p19
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

19. HA Raptor mutant 3

    Plasmid

    #8473

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    ha

    Expression

    Mammalian

    Mutation

    313NWIF316-AAAA (N313A, W314A, I315A, F316A)

    Promoter

    Available since

    May 11, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pUC19-U6-BssgRNA

    Plasmid

    #121957

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    BsCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJT303_SNR52_sgRNA_Can1-3

    Plasmid

    #145066

    Purpose

    Expresses sgRNA targeting Can1-3 site

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    Can1-3 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pALPS puro miR30-IRF9

    Plasmid

    #117159

    Purpose

    Target site AATTATCACAAAGAGGACAGG

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330-H11r1-2-DD-Cas9

    Plasmid

    #85978

    Purpose

    Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    DD-SpCas9
    

    Use

    CRISPR

    Tags

    FKBP12 L106P DD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PX458_ZNF331_2

    Plasmid

    #135740

    Purpose

    Encodes gRNA for 3' target of human ZNF331

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF331 gRNA (ZNF331 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RNF168 C-terminal sgRNA

    Plasmid

    #207100

    Purpose

    pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GAGATGCACAAAGTAAGGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RIF C-terminal sgRNA

    Plasmid

    #207096

    Purpose

    pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AATACTAAATAGAATTTTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. REV7 C-terminal sgRNA

    Plasmid

    #207095

    Purpose

    pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GCTCATAAAGGCAGCTGAGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p8390 LentiCRISPRv2 Neo sgNT-2

    Plasmid

    #221650

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SHLD2 N-terminal sgRNA

    Plasmid

    #207098

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTTTATCAGAAATCATGAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 53BP1 KO sgRNA

    Plasmid

    #207104

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGATTCTCAGCCTGAAAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMLS1272

    Plasmid

    #188891

    Purpose

    Ric-4 sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pALPS puro miR30-TAK1

    Plasmid

    #117162

    Purpose

    Target site AGCGCCCTTCAATGGAGGAAAT

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSV_229

    Plasmid

    #122934

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YBR026C (Marked)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

    Promoter

    [tetO2]4inPgal1

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-EPN-01(ΔL1+L2)

    Plasmid

    #80051

    Purpose

    Expression of EPN-01 mutated in both L domains

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01(deltaPTAP/deltaYP)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PTAP10AAAA, YP37AA

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO-PTEN-shRNA-3001

    Plasmid

    #25639

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-3001 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFUGW-hGtACR2-EYFP

    Plasmid

    #67877

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFUGW-hGtACR1-EYFP

    Plasmid

    #67795

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR1
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR-PpU6P-sgRNA-L3L2

    Plasmid

    #113740

    Purpose

    Gateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR-PpU6P-sgRNA-L1L4

    Plasmid

    #113736

    Purpose

    Gateway entry vector containing attL1 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge