We narrowed to 18,759 results for: nls
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Plasmid#170817PurposeLentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-IDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralExpressionMammalianMutationNAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-Gq-P2A-dTomato
Plasmid#135635PurposeAAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertGq-P2A-dTomato
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TC513
Plasmid#122616PurposePunc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherryDepositorInsertSV40 NLS::rtetR::Zip(+)::SL2::mCherry
ExpressionWormAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSYC-102
Plasmid#74790PurposepCS4+-NLS-EGFP-T2A-mCherry-CAAXDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-EGFP-T2A-mCherry-CAAX
Available sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRD190
Plasmid#163100PurposeExpression of mEos3.2 in eukaryotes to stain the nuclear compartmentDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS_mEos3.2_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-mCherry-P2A-Cre-WPRE
Plasmid#107312PurposeEncoding hSyn-mCherry-CreDepositorHas serviceAAV1InserthSyn-mCherry-P2A-Cre-WPRE
UseAAVExpressionMammalianAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L1-dMb2Cas12a
Plasmid#177683PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeExpressionPlantPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
yWL229 MCP-GFP-eRF3
Plasmid#197053PurposepUBC-NLS-HA-2xMCP-1xGFP-eRF3DepositorPublicationInsertpubc-nls-ha-MCP-GFP-eRF3
UseLentiviralExpressionMammalianPromoterubcAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1_ARF19_2
Plasmid#195914PurposeLevel 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-NLS-tUBQ1DepositorInsertpARF19-PhiC31-NLS-tUBQ1
ExpressionPlantAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P2
Plasmid#195918PurposeLevel 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-tUBQ1DepositorInsertpPP2AA3-PhiC31-NLS-tUBQ1
ExpressionPlantAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P17
Plasmid#195922PurposeLevel 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-tUBQ1DepositorInsertpUBQ10-PhiC31-NLS-tUBQ1
ExpressionPlantAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P22
Plasmid#195925PurposeLevel 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-tUBQ1DepositorInsertp35S-PhiC31-NLS-tUBQ1
ExpressionPlantAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P78
Plasmid#195939PurposeLevel 1 pARF19 reporter : pARF19-mScarlet-NLS-tRBCSDepositorInsertpARF19-mScarlet-NLS-tRBCS
ExpressionPlantAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP
Plasmid#112677PurposeAn AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent proteinDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, and AAV RetrogradeInsertNuclear-localized floxed-mCherry EGFP
UseAAVTagsNuclear localization signalPromoterEF1aAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBK-EE-BE3
Plasmid#85175PurposeExpresses EE-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEE-BE3
ExpressionMammalianPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
NucCKAR
Plasmid#14869DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertC kinase activity reporter
TagsCFP, NLS, and YFPExpressionMammalianAvailable sinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5_NLS-Halo-eGFP-BASU
Plasmid#218449Purposeexpressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integrationDepositorInsertNLS-Halo-eGFP-BASU
ExpressionMammalianPromoterCMVAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD684 VPR-FRB (no NLS)
Plasmid#138851PurposeConstitutive expression of VPR-FRB (no NLS) under the CMV promoter.DepositorInsertVPR-FRB (no NLS)
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD683 VP64-FRB (no NLS)
Plasmid#138850PurposeConstitutive expression of VP64-FRB (no NLS) under the CMV promoter.DepositorInsertVP64-FRB (no NLS)
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD682 VP16-FRB (no NLS)
Plasmid#138849PurposeConstitutive expression of VP16-FRB (no NLS) under the CMV promoter.DepositorInsertVP16-FRB (no NLS)
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEH305 HA NLS SpCas9 3x high affinity MS2 loops
Plasmid#168216PurposeMammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tagDepositorInsertSV40 NLS SpCas9
UseLentiviralTagsHA, SV40 NLS, and nucleoplasmin NLSExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)
Plasmid#107130PurposecGAL-C driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
ExpressionWormPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorPromoterZ3EVAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core
Plasmid#121839PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Gal4-GBP2-IRES-GBP7-p65
Plasmid#50020PurposeMulti-purpose expression of a GFP-dependent transcription factorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGal4DBD-GBP2-IRES-NLS-GBP7-p65AD
UseMulti-purpose expressionPromoterCMV-immediate early promoter and SP6 promoterAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-PBE
Plasmid#98161PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
ExpressionPlantPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS
Plasmid#228400PurposeEncodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasmDepositorInsertNES/PKI-mNeonGreen2x-NLS/FUS
ExpressionMammalianPromoterCMVAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGinB-8X(GGS)-dCas9-FLAG-NLS
Plasmid#81205PurposeCMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpGinB-8X(GGS)-dCas9-FLAG-NLS
ExpressionMammalianPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
JAB3617
Plasmid#190427PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (pro35S::TetR-NLS-19St)DepositorInsertpro35S::TetR-NLS-19St
ExpressionPlantAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3017
Plasmid#190399PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::McbR-NLS-19St)DepositorInsertproG1090::McbR-NLS-19St
ExpressionPlantAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD681 FKBP-ZF43 (no NLS)
Plasmid#138848PurposeConstitutive expression of FKBP-ZF43 (no NLS) under the CMV promoter.DepositorInsertFKBP-ZF43 (no NLS)
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM554 EF1α 3xFLAG-NLS-VP64-intN in TUPV2
Plasmid#161556PurposeConstitutive expression of 3xFLAG-NLS-VP64-intN under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJM555 EF1α 3xFLAG-NLS-DsDed-intN in TUPV2
Plasmid#161557PurposeConstitutive expression of 3xFLAG-NLS-DsDed-intN under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM571 ZF2x6-C 3xFLAG-NLS-VP64-intN in TUPV2
Plasmid#161558PurposeInducible expression of 3xFLAG-NLS-VP64-intN under ZF2x6-C the promoterDepositorInsert3xFLAG-NLS-VP64-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterZF2x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM577 ZF6x6-C 3xFLAG-NLS-DsDed-intN in TUPV2
Plasmid#161563PurposeInducible expression of 3xFLAG-NLS-DsDed-intN under the ZF6x6-C promoterDepositorInsert3xFLAG-NLS-DsDed-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM589 EF1α intN-ZF10-NLS-HA in TUPV2
Plasmid#161565PurposeConstitutive expression of intN-ZF10-NLS-HA under the EF1α promoterDepositorInsertintN-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM583 EF1α 3xFLAG-NLS-VP64-ZF6 in TUPV2
Plasmid#161544PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF6 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF6
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only