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Showing: 1451 - 1500 of 158583 results

  2. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  7. pLAREG

    Plasmid
    #73044
    Purpose
    Inducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.
    Depositor
    David McMillen
    Article
    Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-
    Inserts
    EGFP
    LacI
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 promoter and hybrid dual-mode promoter-see c…
    Available since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73044-plasmid-map-sequence-id-136659

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  12. pLoxP-DHFR-mCherry

    Plasmid
    #70147
    Purpose
    Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion
    Depositor
    David Sibley
    Article
    Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/
    Insert
    a selectable dihydrofolate reductase-thymidylate synthase marker
    Use
    Expression in toxoplasma gondii
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    DHFR 5' UTR
    Available since
    Nov. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    70147-plasmid-map-sequence-id-423191

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  28. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68710-plasmid-map-sequence-id-123992

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  30. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

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  38. pGAPi

    Plasmid
    #127547
    Purpose
    Gateway-based RNAi vector to clone target gene for silencing in tandem with APT transcript segment
    Depositor
    Luis Vidali
    Article
    Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Ubiquitin
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    127547-plasmid-map-sequence-id-249070

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  39. pSY6

    Plasmid
    #90482
    Purpose
    Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum
    Depositor
    Sheng Yang
    Article
    Shao et al Cell Res. 2007 Nov;17(11):963-5. doi: 10
    Insert
    group II intron and ItrA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90482-plasmid-map-sequence-id-178574

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  41. pXY1-spc

    Plasmid
    #90488
    Purpose
    Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052
    Depositor
    Sheng Yang
    Article
    Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90488-plasmid-map-sequence-id-178610

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  42. pDOC-K-glmS

    Plasmid
    #158058
    Purpose
    Donor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Inserts
    glmS homologous region 1
    glmS homologous region 2 + MCS3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158058-plasmid-map-sequence-id-309430

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Showing: 1451 - 1500 of 158583 results