We narrowed to 2,732 results for: SAB;

1. pIS1-Eef25UTR-TOPmut-renilla

   Plasmid

   #38236

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   The first nucleotides of the 5' UTR were cha…

   Promoter

   Endogenous Eef2

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pMXs-RAP2A-GFP

   Plasmid

   #86124

   Purpose

   Retroviral vector expressing RAP2A-GFP-IRES-Bsd

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-63: ACTN2-mEGFP

   Plasmid

   #124607

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFloat.cspA_v1

   Plasmid

   #133071

   Purpose

   Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

   Depositor

   David Sabatini, Kacper Rogala

   Article

   Rogala et al Science. 2019 Oct 25;366(6464):468-475.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   3C-His6 and His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   cspA

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

   Plasmid

   #166611

   Purpose

   Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   EGFP-2A-Tetanus Toxin
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. myc-mTOR (1750-2549 aa)

   Plasmid

   #21747

   Depositor

   David Sabatini

   Article

   Kim et al Mol Cell 2003 Apr;11(4):895-904.

   Insert

   mTOR (1750-2549) (Mtor Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   amino acids 1750-2549

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

7. myc-Rictor

   Plasmid

   #1860

   Purpose

   Please see Addgene item #11367 for an updated version of this plasmid.

   Depositor

   David Sabatini

   Article

   Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

   Insert

   Rictor (RICTOR Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-GK in PLJM60

   Plasmid

   #154207

   Purpose

   Lentiviral vector for stable expression of GK

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   GK (GK Human)
   

   Use

   Lentiviral

   Tags

   N terminal FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-FLAG-C7orf59

   Plasmid

   #42332

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   C7orf59 (LAMTOR4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. myc-mTOR (1271-2008 aa)

    Plasmid

    #21746

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    mTOR (1271-2008) (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    amino acids 1271-2008

    Promoter

    Available since

    Aug. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-Ef1a-DO-EGFP-WPRE-pA

    Plasmid

    #37085

    Purpose

    Expresses EGFP in cells lacking Cre (Cre-Off)

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV hSyn grpLight1.2

    Plasmid

    #175174

    Purpose

    genetically encoded GRP sensor grpLight1.2

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    hSyn-driven grpLight1.2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

    Plasmid

    #166609

    Purpose

    Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-SAMTOR (D190A)

    Plasmid

    #100515

    Purpose

    Expresses HA tagged SAMTOR D190A mutant in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D190A

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-NDUFAF5-HA

    Plasmid

    #86125

    Purpose

    Retroviral vector expressing NDUFAF5-HA-IRES-Bsd

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    NDUFAF5 (NDUFAF5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid

    #70658

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

17. TPI in pCW57_tTA_Blast

    Plasmid

    #154209

    Purpose

    Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    TPI1 (TPI1 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TRE repeats (dox off)

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Ef1a-DO-mCherry-WPRE-pA

    Plasmid

    #37119

    Purpose

    Expresses mCherry in cells lacking Cre (Cre-Off)

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO mouse shRNA 1 raptor

    Plasmid

    #21339

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    raptor shRNA 1 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pMSCV mLST8 myc (aka: GbL-myc)

    Plasmid

    #12580

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    mLST8 (MLST8 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only