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Plasmid#172717PurposeDelivery of guide RNA for prime editingDepositorInsertJ23119-gRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpij23119Available sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas9-ScaligD
Plasmid#125688PurposeGene knockout/in for actinomycetesDepositorInsertstreptomyces codon optimized spCas9, sgRNA casette, and ScaligD
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-dCas9
Plasmid#125687PurposeGene knockdown for actinomycetesDepositorInsertstreptomyces codon optimized spdCas9, sgRNA casette
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MK1283
Plasmid#71428PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …PromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDropVn
Plasmid#184873PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MK1274
Plasmid#71431PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…PromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_T2
Plasmid#184864PurposepSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_T1
Plasmid#184863PurposepSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCBC-DT1T2.2
Plasmid#134752PurposePCR template for sgRNA cloningDepositorInsertsgRNA-AtU6_29p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT1T2.2
Plasmid#134751PurposePCR template for sgRNA cloningDepositorInsertsgRNA-TaU3p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AagRNA
Plasmid#121953PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b tracrRNA/crRNA duplex
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3GB411
Plasmid#134748PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA- OsU3, zCas9-Ubi1Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
ADEPT-pTarget-tetR
Plasmid#238043PurposeModified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterttrBAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
NBS1 C-terminal sgRNA
Plasmid#207092PurposepX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.DepositorInsertTAAAAAGGAGAAGATAACTG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF3A-CDS
Plasmid#136043PurposeUPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)DepositorInsertUPF3A (UPF3A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-VR
Plasmid#62286Purposeexpression of VR sgRNA from the arabinose-inducible promoterDepositorInsertVR sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
T5-sgRNA
Plasmid#113655PurposesgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.DepositorInsertpromoter T5 and sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterT5Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG3H-U6SC
Plasmid#134755PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-35SCAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3K-U6SC
Plasmid#134754PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-35SCAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only