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pDONR221_SLC14A1

Plasmid

#131957

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC14A1 (SLC14A1 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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psgRNA-2xMS2-lacO

Plasmid

#181909

Purpose

Single guide RNA with 2XMS2 loops targeting bacterial lac operator

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

sgRNA-2XMS2-lacO

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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psgRNA-2xMS2-mSAT

Plasmid

#181908

Purpose

Single guide RNA with 2XMS2 loops targeting mouse major satellite repeats

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

sgRNA-2XMS2-mSAT

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

Plasmid

#180314

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 )

Tags

TEV-Strep

Expression

Bacterial

Mutation

SEPT9_i1-i5

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S22A S23A-msfGFP

Plasmid

#180338

Purpose

mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S22A S23A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R10A R15A-msfGFP

Plasmid

#180336

Purpose

mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R10A R15A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLgw NKX2-5-V5-EcoDam

Plasmid

#98597

Purpose

Mammalian DamID lentiviral vector for NKX2-5 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 (Nkx2-5 Mouse)

Use

Lentiviral; Damid

Tags

V5 and Dam (DNA adenine methyltransferase)

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ELK1Y158A

Plasmid

#98628

Purpose

Gateway entry TOPO TA cloning vector ELK1 Y158A

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK1 Y158A (Elk1 Mouse)

Use

Topo ta cloning vector

Mutation

Y158A

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETS1

Plasmid

#98621

Purpose

Gateway entry TOPO TA cloning vector for ETS1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETS1 (Ets1 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

Plasmid

#219565

Purpose

3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

Depositor

Andrea Ventura

Article

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

HygroR_2A_H2B-GFP-N_loxP_mScarlet

Use

Unspecified

Promoter

EF-1a

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP-VPR

Plasmid

#181907

Purpose

Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9-VPR

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1alpha

Plasmid

#181900

Purpose

Fluorescently tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) eGFP

Plasmid

#107505

Purpose

Expresses eGFP, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1gamma

Plasmid

#181902

Purpose

Fluorescently tagged HP1gamma

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1gamma (Cbx3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC14A1_STOP

Plasmid

#161123

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC14A1 (SLC14A1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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alpha actinin delta ABD-GFP

Plasmid

#66935

Purpose

Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

Depositor

Pere Roca-Cusachs, Michael Sheetz

Article

Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Alpha-actinin 1 (ACTN1 Human)

Tags

GFP

Expression

Mammalian

Mutation

actin binding domain (amino acids 30–253) is dele…

Promoter

CMV

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pMECP2-GFP

Plasmid

#181904

Purpose

Fluorescently tagged MECP2, GFP

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

MECP2 (Mecp2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-ELK1

Plasmid

#98604

Purpose

Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK1 (Elk1 Mouse)

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-GFP-mHP1alpha

Plasmid

#181912

Purpose

Bacterial protein expression vector for GFP-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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