We narrowed to 7,219 results for: cas9 plasmid

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pFREE2-SpCas9-sgRNA

Plasmid

#179582

Purpose

Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

Cas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFREE2-xCas9-sgRNA

Plasmid

#179579

Purpose

Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

xCas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFREE2-eSpCas9-sgRNA

Plasmid

#179580

Purpose

Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

eSpCas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CcuCas9

Plasmid

#192127

Purpose

Expresses CcuCas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

CcuCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp2Cas9

Plasmid

#192126

Purpose

Expresses Hsp2Cas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1Cas9

Plasmid

#192125

Purpose

Expresses Hsp1Cas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.pU1a-SpCas9

Plasmid

#121507

Purpose

Expresses codon-optimized SpCas9 in mammalian cells.

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

SpCas9

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

U1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR1-P2-SpCas9

Plasmid

#232354

Purpose

The plasmid pBBR1-P2-SpCas9 employs pBBR1MCS-2 as the vector, with Cas9 protein derived from S. pyogenes and the stationary phase promoter P2 sourced from S. marcescens HBQA7.

Depositor

Yujie Cai

Article

Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-

Inserts

Cas9
sacB

Use

CRISPR

Promoter

promoter P2 from S. marcescens HBQA7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

Plasmid

#223111

Purpose

pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9-P2A-mTagBFP2

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-mTagBFP2

Expression

Mammalian

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-P2A-EGFP (MSP2582)

Plasmid

#223067

Purpose

pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized SpCas9-P2A-EGFP

Use

CRISPR

Tags

NLS(SV40)-3xFLAG-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

Plasmid

#209199

Purpose

Mutagenesis of Kcnma1

Depositor

Larry Zweifel

Article

Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

Insert

Kcnma1 (Kcnma1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-SpCas9

Plasmid

#104588

Purpose

AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Has Service

AAV8

Insert

Streptococcus pyogenes Cas9

Use

AAV and CRISPR

Tags

Myc

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-HF1

Plasmid

#108301

Purpose

pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N

Plasmid

#80930

Purpose

Expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-eSpCas9(1.1)

Plasmid

#108292

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-SpCas9-HF1

Plasmid

#108293

Purpose

pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-TadCDd_gRNA

Plasmid

#202563

Purpose

P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

AAV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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