Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,078 results for: control gRNA

Clear keywords
Showing: 581 - 600 of 1078 results

  4. pBFC1207

    Plasmid
    #186694
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Publication
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186694-plasmid-map-sequence-id-371385

    Enlarge

  6. AAV-KrasWT/sgKras/Cre

    Plasmid
    #99848
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Publication
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99848-plasmid-map-sequence-id-193786

    Enlarge

  8. pdCas9-M-3F2

    Plasmid
    #73428
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73428-plasmid-map-sequence-id-135328

    Enlarge

  16. pSL1103

    Plasmid
    #129526
    Purpose
    pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation
    Depositor
    Scott Lindner
    Publication
    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9
    Use
    Plasmodium, rodent-infectious species
    Tags
    NLS, 1xHA
    Available since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    129526-plasmid-map-sequence-id-256135

    Enlarge

  20. Lentiviral nontargeting shRNA GFP

    Plasmid
    #155285
    Purpose
    Lentiviral expression of nontargeting shRNA, GFP expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155285-plasmid-map-sequence-id-311904

    Enlarge

Showing: 581 - 600 of 1078 results