We narrowed to 9,397 results for: bli

1. pMAL-c5X-MAP

   Plasmid

   #66999

   Purpose

   Expresses malE-MAP in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-MAP
   

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB700-mouse-ACTC1-Puro

   Plasmid

   #128326

   Purpose

   SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

   Depositor

   Alejandro Chavez

   Insert

   gRNA against mouse ACTC1 for activation (Actc1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SUV420H2

   Plasmid

   #32864

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SUV420H2 (KMT5C Human)
   

   Tags

   His tag (with TEV protease)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. CAMK2D

   Plasmid

   #38987

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2D (CAMK2D Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pIYC08

   Plasmid

   #64743

   Purpose

   Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

   Depositor

   Jens Nielsen

   Article

   Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

   Insert

   ADH2, ALD6, acsL641P, ERG10
   

   Expression

   Yeast

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

   Plasmid

   #105922

   Purpose

   AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR

   Depositor

   Marcella Patrick

   Inserts

   mKate2
   IRES-Multiple cloning site
   

   Use

   AAV

   Mutation

   codon optimized

   Promoter

   CBh and same CBh promoter as mKate2

   Available Since

   Feb. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pdCas9-tdTomato-VPR

   Plasmid

   #232632

   Purpose

   VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer

   Depositor

   Karsten Rippe

   Insert

   dCas9-tdTomato-VPR
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

   Plasmid

   #51095

   Purpose

   Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   ChIEF(E162A/T198C)
   

   Use

   AAV

   Tags

   P2A-dTomato

   Expression

   Mammalian

   Mutation

   E162A/T198C

   Promoter

   EF1a

   Available Since

   May 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-ACE2

   Plasmid

   #158438

   Purpose

   Gateway Cloning compatible entry vector for the human ACE2 gene.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4R3H

    Plasmid

    #64652

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    April 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP

    Plasmid

    #185696

    Purpose

    AAV vector expressing cre-dependent mem-mScarlet, T2A, Synaptophysin-EGFP driven by Dlx promoter

    Depositor

    Lynette Lim

    Insert

    mScarlet, T2A, Synaptophysin-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    mScarlet palmitoylation, Synaptophysin fused to E…

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid

    #72639

    Purpose

    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain

    Depositor

    Joshua Bonkowsky

    Insert

    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    

    Use

    Gateway

    Mutation

    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. BRDT

    Plasmid

    #38898

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRDT (BRDT Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid

    #90473

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Insert

    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EP300

    Plasmid

    #39018

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    EP300 (EP300 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pTXB1-FIS

    Plasmid

    #51564

    Purpose

    Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    FIS
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. FH

    Plasmid

    #38978

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FH (FH Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6P1-DEST-Strep2

    Plasmid

    #119753

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.

    Depositor

    Andrew Jackson, Martin Reijns

    Type

    Empty backbone

    Tags

    GST and Strep-tag II fusion tag

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69546

    Purpose

    Tet-On system expressing MyoD-2A-GFP-2xNLS.

    Depositor

    Charles Gersbach

    Inserts

    eGFP
    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Mutation

    Removed stop codon to fuse it to the 2A-eGFPnls i…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGreenII Tfs-494::LUC

    Plasmid

    #44467

    Purpose

    Expresses a luciferase gene disrupted by an AtCRU3 TALEN Target 494 site and a frame-shift mutation

    Depositor

    Roger Hellens, Ross Johnson

    Insert

    A luciferase gene disrupted by an AtCRU3 TALEN Target 494 site and a frame-shift mutation
    

    Use

    Luciferase; Plant transformation

    Mutation

    A disruption was made between the first and secon…

    Promoter

    35S Promoter

    Available Since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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