We narrowed to 11,789 results for: nsf

1. pAKW.111A-SETD7

   Plasmid

   #154052

   Purpose

   Expresses SETD7 (Y305F) in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   Histone-lysine N-methyltransferase SETD7 (SETD7 Human)
   

   Expression

   Bacterial

   Mutation

   changed tyrosine 305 to phenylalanine

   Promoter

   pTet*

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-miRFP720

   Plasmid

   #197213

   Purpose

   Expresses the protein of miRFP720 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   miRFP720
   

   Use

   AAV

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-DIO-NaChBac-dTomato

   Plasmid

   #236252

   Purpose

   Cre dependent NaChBac-TdTomato expression

   Depositor

   Janelia Viral Tools

   Article

   Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

   Insert

   NaChBac-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-iRFP

   Plasmid

   #64887

   Purpose

   Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

   Depositor

   Tomomi Ichinose

   Article

   Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

   Insert

   iRFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW-Cas9-Blast

   Plasmid

   #83481

   Purpose

   Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

   Depositor

   Mohan Babu

   Inserts

   SpCas9
   Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   Tight Tre and human phosphoglycerate kinase 1 pro…

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. paavCAG-iCre

   Plasmid

   #51904

   Purpose

   For AAV expression of iCre recombinase

   Depositor

   Jinhyun Kim

   Article

   Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

   Insert

   improved Cre recombinase
   

   Use

   AAV and Cre/Lox

   Promoter

   CAG

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-gRNA-CBh-mCherry

   Plasmid

   #91947

   Purpose

   Expression of empty gRNA cassette and mCherry

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Insert

   mCherry
   

   Use

   AAV

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-ConVERGD-(HA)hM3Dq-W3SL

   Plasmid

   #218758

   Purpose

   Provides AND intersectional (Cre+Flp) expression of HA-hM3Dq

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   hM3Dq
   

   Use

   AAV

   Tags

   HA

   Promoter

   hSyn

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. rtTA-N144

   Plasmid

   #66810

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

   Depositor

   Andrew Yoo

   Article

   Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

   Insert

   Reverse tetracycline-controlled transactivator
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human EF1a

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Expression

    Bacterial

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-DIO-SPOTlight-U2GCR

    Plasmid

    #231889

    Purpose

    Cre-dependent reporter to measure ISR state-dependent protein synthesis

    Depositor

    Nicole Calakos

    Insert

    DIO-SPOTlight-U2GCR
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET21a-MTF-His

    Plasmid

    #124133

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionyl-tRNA formyltransferase (fmt Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-R-Creon shRNA[Control]

    Plasmid

    #180775

    Purpose

    This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

    Depositor

    Eun Mi Hwang

    Article

    Kim et al J Neurosci Methods. 2021 Dec 21:109452.

    Type

    Empty backbone

    Use

    AAV, Cre/Lox, and RNAi

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-Camk2-NES-Twitch-GR

    Plasmid

    #173044

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-GR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-shRNA-ctrl

    Plasmid

    #85741

    Purpose

    shRNA ctrl

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA ctrl
    

    Use

    AAV

    Tags

    EYFP

    Available Since

    April 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

    Plasmid

    #50460

    Purpose

    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-Camk2-NES-Twitch-NR

    Plasmid

    #173043

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-NR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid

    #50476

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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