164,802 results

1. pEHIShqprt

   Plasmid

   #92166

   Purpose

   Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

   Depositor

   Huanting Liu, James Naismith

   Article

   Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

   Insert

   Quinolinate Phosphoribosyltransferase (QPRT Human)
   

   Use

   Tags

   TEV protease -cleavable N-terminal 6His

   Expression

   Bacterial

   Mutation

   changed Asp 2 to Asn to create BspHI site

   Promoter

   T7

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-DpnIR

   Plasmid

   #165529

   Purpose

   Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   DpnI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV.GFAP.Cre.WPRE.hGH

   Plasmid

   #105550

   Purpose

   AAV expression of Cre from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV PHP.eB and AAV5

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162381

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CAG

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEff

   Plasmid

   #97423

   Purpose

   Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

   Depositor

   Nikolai Ravin

   Article

   Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPI

   Plasmid

   #12254

   Purpose

   2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EMCV IRES-EGFP cassette

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR322-PVCpnf17-22

   Plasmid

   #198272

   Purpose

   Unmodified pPayload; contains WT PVC payloads/regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf17-22
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30a-Tn5

   Plasmid

   #127917

   Purpose

   Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

   Depositor

   Silin Zhong

   Article

   Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

   Insert

   Tn5
   

   Use

   Tags

   Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304 Flag-mCherry-eEF1A1_IRES-GFP

   Plasmid

   #198383

   Purpose

   eEF1A1 fluorescent reporter

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   eEF1A1 (EEF1A1 Human)
   

   Use

   Lentiviral

   Tags

   Flag-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB513

    Plasmid

    #74855

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-KDEL_TNF-SBP-EGFP

    Plasmid

    #65278

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB504

    Plasmid

    #74846

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15-UbE2W

    Plasmid

    #15809

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2W (UBE2W Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-hCDC6p-Venus-puro

    Plasmid

    #212666

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hCDC6p

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3XFLAG-VP64-SadCas9-NLS-VP64

    Plasmid

    #135338

    Purpose

    3xFLAG 2xVP64 SadCas9 for single guide cloning

    Depositor

    Ronald Cohn

    Article

    Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

    Insert

    SadCas9
    

    Use

    Tags

    3xFLAG-VP64-SV40 NLS and NLS-VP64

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207180

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Beta3

    Plasmid

    #140988

    Purpose

    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta3 (GNB3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-neo-PP1-Beta

    Plasmid

    #171269

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 beta catalytic subunit

    Depositor

    Steven Balk

    Insert

    3x-Flag-tagged human PP1 beta catalytic subunit (PPP1CB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermEp* promoter from S. erythraea

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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